We narrowed to 31,810 results for: Ide

1. CMV-O-GECO1

   Plasmid

   #46025

   Purpose

   Orange intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   O-GECO1
   

   Expression

   Mammalian

   Mutation

   R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…

   Promoter

   CMV

   Available Since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEED-mScarlet3:ALFA

   Plasmid

   #219641

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet:ALFA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLM3705

   Plasmid

   #47754

   Purpose

   Expresses mammalian cell codon-optimized dCas9-VP64

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   codon optimized dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9 (catalytically i…

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMD.RVG.CVS24-N2c

   Plasmid

   #19712

   Depositor

   Manfred Schubert

   Article

   Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

   Insert

   Rabies virus CVS24-N2c glycoprotein
   

   Expression

   Mammalian

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-FLAG-CaMKIIB

   Plasmid

   #186826

   Purpose

   Expresses FLAG-CaMKIIB in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2b (Camk2b Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2-scr

   Plasmid

   #169795

   Purpose

   Expresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1..

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   Scrambled guide RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEX-A-U6-gRNA

   Plasmid

   #65626

   Purpose

   Empty vector for the expression U6 driven gRNA

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   U6-gRNA
   

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2CT-His-MBP-Lsh_C2c2_WT

   Plasmid

   #83487

   Purpose

   Bacterial expression of WT L. shahii C2c2 CRISPR effector

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lsh_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAT15489_dABE8e

   Plasmid

   #174127

   Purpose

   plasmid expressing ABE8e with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dABE8e
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3NES-CS(G)-tTA-CS(G)-3NES

    Plasmid

    #137836

    Purpose

    Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    3NES-CD(G)-tTA-CS(G)-3NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ubiq-Cas9.874W

    Plasmid

    #112686

    Purpose

    Express hSpCas9 under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    Ubiquitin-63E promoter

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDAS12125_PEAR-GFP

    Plasmid

    #177178

    Purpose

    PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMarVF1

    Plasmid

    #99579

    Purpose

    Minitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114

    Depositor

    Mark Mandel

    Article

    Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

    Insert

    V. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
    

    Use

    Suicide vector in v. fischeri es114

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPCK

    Plasmid

    #133128

    Purpose

    a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    PEP-carboxykinase gene
    

    Expression

    Bacterial

    Promoter

    PEP-carboxykinase promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTM1_VP35_ZEBOV

    Plasmid

    #68121

    Purpose

    Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    VP35 ZEBOV (VP35 )
    

    Use

    T7

    Promoter

    T7

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJAT11

    Plasmid

    #204290

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-mFzd3

    Plasmid

    #42255

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 3 (Fzd3 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

    Plasmid

    #168173

    Purpose

    Expression of BeCyclOp in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

    Insert

    BeCyclOp (codon optimized for C. elegans)
    

    Expression

    Worm

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCLC3sGFP

    Plasmid

    #52423

    Purpose

    mammalian expression of rat CLC3 short transcript fused to EGFP

    Depositor

    Steven Weinman

    Article

    Li et al Am J Physiol Cell Physiol. 2002 Jun;282(

    Insert

    CLC3 (Clcn3 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPR V2 sgIREB2_2

    Plasmid

    #169886

    Purpose

    Disrupt IREB2

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting IREB2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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