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pET28-hisSumo-KE-Des27.7_ModularAssembly&activeSite

Plasmid

#248013

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&ActiveSite design)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly&activeSite

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_WT-LGP2 (codon optimized)

Plasmid

#248163

Purpose

Expresses full-length human LGP2 in E. coli

Depositor

Smita Patel

Article

Lee et al Nucleic Acids Res. 2024 Jan 11;52(1):355

Insert

DExH-box helicase 58 (DHX58) (DHX58 Human)

Tags

6xHis, SUMO

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28 His-SUMO-CHMP2B

Plasmid

#232018

Purpose

Bacterial expression of His and SUMO tagged CHMP2B

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

6x His, SUMO

Expression

Bacterial

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-His6-UROD-N367A

Plasmid

#236740

Purpose

Protein overexpression of human uroporphyrinogen decarboxylase N367A

Depositor

Christina Woo

Article

Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

Insert

Uroporphyrinogen decarboxylase human N367A (UROD Human)

Tags

His6

Expression

Bacterial

Mutation

N367A

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-His6-GFP-UROD

Plasmid

#236741

Purpose

Protein overexpression of eGFP-human uroporphyrinogen decarboxylase

Depositor

Christina Woo

Article

Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

Insert

eGFP-Uroporphyrinogen decarboxylase human (UROD Human, Synthetic)

Tags

His6 and eGFP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-SUMO-FLAG-TeKaiC

Plasmid

#229222

Purpose

For expression of T. elongatus KaiC protein

Depositor

Andy LiWang

Insert

kaiC (kaiC T. elongatus)

Tags

SUMO-FLAG

Expression

Bacterial

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-SUMO-SeKaiB-FLAG

Plasmid

#229219

Purpose

For expression of S. elongatus KaiB protein

Depositor

Andy LiWang

Insert

kaiB (kaiB S. elongatus)

Tags

SUMO

Expression

Bacterial

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-mScarlet-i

Plasmid

#191196

Purpose

Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-SAV

Plasmid

#191197

Purpose

Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-mScarlet-i

Plasmid

#191199

Purpose

Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-SAV

Plasmid

#191200

Purpose

Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.86b.8D2Q.Tau2N4R

Plasmid

#226400

Purpose

Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 8D2Q mutant 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau (MAPT Human, synthetic)

Tags

His.3C.86b

Expression

Bacterial

Mutation

8D2Q.Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.86b.20D3Q.Tau2N4R

Plasmid

#226401

Purpose

Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C.86b

Expression

Bacterial

Mutation

20D3Q, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-PKP3(305-797)

Plasmid

#202599

Purpose

Expresses ARM domain of plakophilin3

Depositor

Tina Izard

Article

Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

Insert

plakophilin3 (PKP3 Human)

Tags

His8 tag with precision protease cleavage site

Expression

Bacterial

Mutation

Deleted amino acids 1 to 304

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

Plasmid

#187708

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-380

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W3-W4-HECT (492)

Plasmid

#187709

Purpose

Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-IRSp53(pLxIS)

Plasmid

#221336

Purpose

Overexpression of IRSp53 pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 pLxIS domain (Baiap2 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-MAVS(pLxIS)

Plasmid

#221335

Purpose

Overexpression of MAVS pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS pLxIS domain (Mavs Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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