We narrowed to 31,790 results for: son
-
Plasmid#205235PurposeExpresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cellsDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
TagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir2-LNS2
Plasmid#220086PurposeC-terminal PA binding domain of Nir2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPITPNM1 (PITPNM1 Human)
TagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 896-1216PromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNESx2-EGFP-C1-PABDx2-Spo20
Plasmid#220082PurposePA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear exportDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpo20 (SPO20 Budding Yeast)
TagsX. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…ExpressionMammalianMutationamino acids 51-91, tandem dimerPromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNKX2-5-T2A-GFP-PGK-Puro
Plasmid#203607PurposeDonor plasmid to create 3' knockin of NKX2-5 with T2A-GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
UseHuman targetingAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-hCD59-T2A-GFP
Plasmid#205460Purposelentiviral plasmid for expression of human CD59DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthCD59 (CD59 Human)
ExpressionMammalianPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase
Plasmid#196185PurposeCo-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-HisDepositorInsertsTagsGST, HA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
3322_pETcon_SARS2_Omicron-BA2
Plasmid#184409Purposeyeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBDDepositorInsertSARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastMutationG339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GLI1_pcDNA6.2/EmGFP-Bsd
Plasmid#176968PurposeMammalian expression vector encoding GLI1 and EmGFP-BsdDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGLI1 (GLI1 Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-LRP4 (extracellular) [N207/27R]
Plasmid#177513PurposeMammalian Expression Plasmid of anti-LRP4 (extracellular) (Mouse). Derived from hybridoma N207/27.DepositorInsertanti-LRP4 (extracellular) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)
ExpressionMammalianPromoterDual CMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Anti-Nav1.7 Na+ channel [N68/6R]
Plasmid#177566PurposeMammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6.DepositorInsertanti-Nav1.7 Na+ channel (Homo sapiens) recombinant mouse monoclonal antibody (SCN9A Mouse)
ExpressionMammalianPromoterDual CMVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-CASK [K56A/50R]
Plasmid#177443PurposeMammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/50.DepositorInsertanti-CASK (Homo sapiens) recombinant mouse monoclonal antibody (CASK Mouse)
ExpressionMammalianPromoterDual CMVAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE
Plasmid#172908PurposeAAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequenceDepositorHas serviceAAV1InsertAce2N-4AA-mNeon-ST A122D
UseAAV and Cre/LoxTagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterhSynapsin1Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer
Plasmid#176589PurposeEndogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Chesapeake Bay) locus
ExpressionBacterialMutationTruncated CRISPR array to two direct repeats, rep…Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a_3xFlag-nsp14 (SARS-CoV-2)
Plasmid#169159PurposeTo express SARS-CoV-2 nsp14 in E. coliDepositorInsert3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
Tags3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…ExpressionBacterialMutationCodon optimised for E. coliPromoterT7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-FLAG-NEK9T210A
Plasmid#168270PurposeExpresses kinase-dead NEK9 mutant in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNEK9 (NEK9 Human)
TagsFLAGExpressionMammalianMutationchanged threonine 210 to alanineAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
SL2-sgTelo-MTSa/BFP/pdCas9-C1
Plasmid#162760PurposeExpressing dCas9 and sgRNA containg MTSa targeting telomeresDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9 and sgRNA(SL2-sgTelo-MTSa)
ExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE
Plasmid#139095PurposeExpresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertKRAB-dSpCas9-NG
UseCRISPR and LentiviralExpressionMammalianMutationD10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_WW-PolyA
Plasmid#112290PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDADepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with mutations in the WW domain…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP
Plasmid#121448PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotationsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRFPnols-PQR-ZeocinR (ACTB Human)
UseCRISPRExpressionMammalianAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUDP044
Plasmid#101168PurposepUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only