Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,186 results for: egf

Clear keywords
Showing: 9621 - 9640 of 15186 results

  1. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Publication
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

    Enlarge

  3. p458 VRER

    Plasmid
    #101728
    Purpose
    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VRER
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutation…
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101728-plasmid-map-sequence-id-196591

    Enlarge

  6. pCAG-hCas9D10A

    Plasmid
    #52101
    Purpose
    The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter
    Depositor
    Izuho Hatada
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hCas9D10A
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CAG
    Available since
    April 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  8. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid
    #131313
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    131313-plasmid-map-sequence-id-254655

    Enlarge

  15. GFP-polyQ74-NLS

    Plasmid
    #231892
    Purpose
    Forms nuclear HTT partial exon 1 Q74-NLS aggregates
    Depositor
    Lukas Kapitein
    Publication
    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT partial exon 1 Q74-NLS (HTT Human)
    Tags
    EGFP and SV40 NLS
    Expression
    Mammalian
    Mutation
    CAG repeat expansion
    Available since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    231892-plasmid-map-sequence-id-461348

    Enlarge

  16. pSSA-RPG

    Plasmid
    #85932
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Publication
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Expression
    Mammalian
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    85932-plasmid-map-sequence-id-172472

    Enlarge

  17. hHS1-M7A

    Plasmid
    #159170
    Purpose
    Human codon-optimized cytosolic labile heme reporter
    Depositor
    Amit Reddi
    Publication
    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hHS1-M7A
    Expression
    Mammalian
    Mutation
    Mutated Met 7 of the cyt b562 module of hHS1 to A…
    Promoter
    CMV
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    159170-plasmid-map-sequence-id-311541

    Enlarge

  18. Cx30-msfGFP

    Plasmid
    #69019
    Purpose
    Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Publication
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cx30 (GJB6 Human)
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Promoter
    c
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    69019-plasmid-map-sequence-id-127981

    Enlarge

Showing: 9621 - 9640 of 15186 results