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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 9621 - 9640 of 18384 results

  1. pBHM2647

    Plasmid
    #229989
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229989-plasmid-map-sequence-id-461139

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  2. pBHM2648

    Plasmid
    #229990
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229990-plasmid-map-sequence-id-461146

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  3. pBHM2649

    Plasmid
    #229991
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229991-plasmid-map-sequence-id-461155

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  4. pX459_MCM2_sgRNA

    Plasmid
    #232730
    Purpose
    Generates MCM2 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    MCM2 (MCM2 Human)
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232730-plasmid-map-sequence-id-465451

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  7. pLV_CAG_Gag∆MA

    Plasmid
    #212633
    Purpose
    Lentivirus vector to express MLV Gag with truncated MA domain
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Deletion of MA domain (amino acids 2-215)
    Promoter
    CAG
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    212633-plasmid-map-sequence-id-422303

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  8. 1GFP-His-GFP-TEV-cs-ATG3

    Plasmid
    #197472
    Purpose
    Bacterial Expression of human ATG3
    Depositor
    James Hurley
    Insert
    ATG3 (ATG3 Human)
    Tags
    His-GFP
    Expression
    Bacterial
    Available Since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    197472-plasmid-map-sequence-id-389158

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  9. pAG1guide_RUBY_EggCas9

    Plasmid
    #225983
    Purpose
    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants
    Depositor
    Imran Siddiqi
    Insert
    Guide RNA against AtAGAMOUS1
    Use
    CRISPR
    Expression
    Plant
    Promoter
    35s promoter to Drive RUBY and DD45p to drive Cas9
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225983-plasmid-map-sequence-id-448020

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  11. pNHA_SBV_Gc

    Plasmid
    #208933
    Purpose
    Expresses N-terminally HA-tagged Schmallenberg orthobunyavirus Gc protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Gc
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 1682 bp from 5' end, deleted 378 bp …
    Promoter
    CMV
    Available Since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208933-plasmid-map-sequence-id-419021

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  12. cacna2d1 R241A D491A HA pcDNA3

    Plasmid
    #206109
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Expression
    Mammalian
    Mutation
    R241A D491A
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206109-plasmid-map-sequence-id-415382

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  13. mEos3.2trLAT-2P

    Plasmid
    #203748
    Purpose
    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLAToC27AC30A (Lat Mouse)
    Tags
    ER translocation signal - mEos3.2
    Expression
    Mammalian
    Mutation
    C27A, C30A
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203748-plasmid-map-sequence-id-403342

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  14. IDG_CHRNB1_OE_1

    Plasmid
    #161659
    Purpose
    Overexpresses CHRNB1
    Depositor
    Mike McManus
    Insert
    CHRNB1 (CHRNB1 Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    161659-plasmid-map-sequence-id-319640

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  16. pT7-V5-SBP-C1-HsDIS3_Z

    Plasmid
    #148137
    Purpose
    Mammalian Expression of HsDIS3
    Depositor
    Elisa Izaurralde
    Insert
    HsDIS3 (DIS3 Human)
    Expression
    Mammalian
    Mutation
    L760F compared to the sequence given by isoform 2…
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148137-plasmid-map-sequence-id-453676

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  17. pLNHA-C1-HseIF4AI_K

    Plasmid
    #146731
    Purpose
    Mammalian Expression of HseIF4AI
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4AI (EIF4A1 Human)
    Expression
    Mammalian
    Mutation
    a non silent mutation (A200V) and 3 silent mutati…
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    146731-plasmid-map-sequence-id-357210

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  18. pGEX4T1-WWP1F792D

    Plasmid
    #179328
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1 F792D
    Depositor
    Arthur Haas
    Insert
    WWP1 F792D (WWP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    F792D mutation; Codon Optimized for expression in…
    Available Since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179328-plasmid-map-sequence-id-358707

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  19. pCDZ2FE_Rev3_1873-2025_4PA

    Plasmid
    #105673
    Purpose
    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein with mutations abolishing Rev7 binding
    Depositor
    Thanos Halazonetis
    Insert
    Rev3L (REV3L Human)
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Mutations: P1880A, P1885A, P1996A, P2001A
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105673-plasmid-map-sequence-id-203204

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  20. SDCBPA-c010

    Plasmid
    #175482
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175482-plasmid-map-sequence-id-353168

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Showing: 9621 - 9640 of 18384 results