161,621 results

1. Aldh1L1 scFv [N103/31]

   Plasmid

   #190522

   Purpose

   Mammalian Expression of Aldh1L1 scFV. Derived from hybridoma N103/31.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Aldh1L1 (Rattus norvegicus) recombinant scFV (Aldh1l1 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5xQUAS-ZsGreen-P2A, α-cry:mCherry

   Plasmid

   #180477

   Purpose

   Inducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherry

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Inserts

   5x QUAS-ZsGreen-P2A
   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Epac-S-H170

   Plasmid

   #170352

   Purpose

   mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCKan-yibDp-ald*-alaE

   Plasmid

   #134939

   Purpose

   Low phosphate inducible alanine dehydrogenase and alanine exporter

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   alanine dehydrogenase and L-alanine exporter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. R777-E202 Hs.PRKAG2-nostop

   Plasmid

   #70486

   Purpose

   Gateway ORF clone of human PRKAG2 [NM_024429.1] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   PRKAG2 (PRKAG2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p414GPD_Hsp82viiB

   Plasmid

   #61709

   Purpose

   Yeast plasmid with Hsp82 heterodimer construct.

   Depositor

   Dan Bolon

   Article

   Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

   Insert

   Hsp82viiB (HSP82 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Heteromeric coiled coil inserted after amino acid…

   Promoter

   GPD1

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHOHO2_Hsp82viiA

   Plasmid

   #61708

   Purpose

   Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.

   Depositor

   Dan Bolon

   Article

   Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

   Insert

   Hsp82viiA (HSP82 Budding Yeast)
   

   Use

   Yeast integration vector targeting inserts to the…

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Heteromeric coiled coil inserted after amino acid…

   Promoter

   TEF1

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-Con/Fon-mCherry (AAV8)

   Viral Prep

   #137132-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-mCherry (#137132). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-mCherry plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   mCherry (Cre and Flp-dependent)

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

   Plasmid

   #221359

   Purpose

   Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA-HaloGFP-Na2.4

    Plasmid

    #228471

    Purpose

    Mammalian expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

    Depositor

    Robert Campbell

    Article

    Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

    Insert

    HaloGFP-Na2.4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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