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p2,0_3M5M_luciferase

Plasmid

#135475

Purpose

Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

Marburg virus minigenome, luciferase reporter

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHN924

Plasmid

#178885

Purpose

Expression of GST-His8-HRV3C

Depositor

Kazuhiro Abe

Insert

human rhinovirus 3C protease

Tags

GST, His8

Expression

Bacterial

Promoter

T7

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNL3.2.CMV.NLuc-PEST.Hygro

Plasmid

#209998

Purpose

In mammalian cells expresses NanoLuc mRNA with no TH as a control. Also expresses a hygromycin resistance marker.

Depositor

Kevin Weeks

Article

Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

Inserts

NanoLuc
Hygromycin resistance marker

Expression

Mammalian

Promoter

CMV and SV40

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB510

Plasmid

#74852

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB510

Plasmid

#74852

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-4591NES

Plasmid

#138377

Purpose

Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-ROCK

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-4591NES

Plasmid

#138377

Purpose

Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-ROCK

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_WT

Plasmid

#81925

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KEAP1 (KEAP1 Human)

Use

Gateway Cloning

Mutation

C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_WT

Plasmid

#81925

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KEAP1 (KEAP1 Human)

Use

Gateway Cloning

Mutation

C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBEVY-pT231 scFv-GFP

Plasmid

#218827

Purpose

Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

pT231 tau scFv

Expression

Yeast

Promoter

GAL1-10

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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