166,818 results

1. pIS1-Eef25UTR-renilla

   Plasmid

   #38235

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2 5'UTR / Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Eef2 5' UTR fused to renilla ORF

   Promoter

   Endogenous Eef2

   Available Since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-neo (1795)

   Plasmid

   #41000

   Depositor

   Jonathon Pines

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. opto-Rac1

   Plasmid

   #135396

   Purpose

   Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3

   Insert

   opto-Rac1
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-PIK3CA

   Plasmid

   #116771

   Purpose

   Lentiviral expression of PIK3CA

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CVB3 IRES

   Plasmid

   #189968

   Purpose

   Part 2 plasmid to introduce the CVB3 IRES into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   CVB3 IRES
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH2B-mNG2(11)

   Plasmid

   #206043

   Purpose

   Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.

   Depositor

   Alisa Piekny

   Article

   Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

   Insert

   H2B gene fused to the mNG2(11) fragment
   

   Use

   Synthetic Biology

   Tags

   H2B-mNG2(11)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 4xmts-mScarlet-I

   Plasmid

   #98818

   Purpose

   In vivo visualization of mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   mts
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_047

   Plasmid

   #107145

   Purpose

   measures Cas9 activity

   Depositor

   David Root

   Insert

   GFP + sgRNA for GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid

    #47549

    Purpose

    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    Empty sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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