We narrowed to 18,346 results for: Mut

1. pcDNA3.1-hRIPK1-Flag-delC

   Plasmid

   #112493

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of C terminus (AA290-671 deleted)

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Phage-PGK-GFP-T(WT)

   Plasmid

   #181736

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available Since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

   Plasmid

   #50468

   Purpose

   Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Insert

   rM3D-IRES-mCitrine
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   CaMKIIa

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRL-TK let7 A

   Plasmid

   #11324

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

   Insert

   2 binding sites for let-7a miRNA
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Mutation A from figure 4A of paper.

   Promoter

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pmSod1-G93A-N’His

   Plasmid

   #117705

   Purpose

   mutation on stability/solubility of SOD1

   Depositor

   Elizabeth Fisher

   Insert

   Sod1 (Sod1 Mouse)
   

   Use

   Tags

   His tag with Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   G93A

   Promoter

   T7

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hRIPK1-Flag-delN

   Plasmid

   #112492

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of N terminus (AA1-274 deleted)

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p87AAVEGFP hp47phox (S303/304/328D)

   Plasmid

   #112250

   Purpose

   mutant p47phox

   Depositor

   Simone Di Giovanni

   Insert

   p47phox (NCF1 Human)
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mutation

   S303/304/328D

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-hRIPK1-Flag-delDD

   Plasmid

   #112494

   Purpose

   Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of DD domain (AA584-671 deleted)

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. XClone_Cas9_KRAB_T2A_BFP

   Plasmid

   #141208

   Purpose

   Tunable and temporal expression control of dCas9

   Depositor

   Rory Johnson

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMCP-tag (m) Vector

    Plasmid

    #101127

    Purpose

    Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of MCP-tag.

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Use

    Tags

    MCP-tag (Mutant ACP- tag)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pMCP-GPI Control Plasmid

    Plasmid

    #101132

    Purpose

    Expression of MCP-Tagged GPI anchor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    GPI
    

    Use

    Tags

    MCP-tag (Mutant ACP- tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pAM1956

    Plasmid

    #40251

    Depositor

    Susan Golden

    Article

    Yoon et al Science. 1998 Oct 30;282(5390):935-8.

    Insert

    GFPmut2
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500

    Plasmid

    #45779

    Depositor

    Richard Murray, Vincent Noireaux

    Insert

    sigma factor 28
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available Since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRL-TK let7 B

    Plasmid

    #11325

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

    Insert

    2 binding sites for let-7a miRNA
    

    Use

    Luciferase

    Tags

    Rr-luc

    Expression

    Mutation

    Mutation B from figure 4A of paper.

    Promoter

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pET-24a-STE2

    Plasmid

    #133430

    Purpose

    Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Both native Cys (C59S/C252A) have been mutated. S…

    Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSET QUEEN7mu R92A

    Plasmid

    #129367

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN7mu R92A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET QUEEN7mu R99A

    Plasmid

    #129370

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN7mu R99A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK GFP Paxillin Y31A

    Plasmid

    #50530

    Depositor

    Kenneth Yamada

    Insert

    Paxillin (PXN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y31A

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. GST-E6AP C833A

    Plasmid

    #13465

    Depositor

    Michael Greenberg

    Insert

    E6-AP (UBE3A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C833A; active site Cys mutated to encode Ala

    Promoter

    Available Since

    March 6, 2007

    Availability

    Academic Institutions and Nonprofits only

20. Phage-PGK-FLAG-HA-T(WT)

    Plasmid

    #181737

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Promoter

    Available Since

    Aug. 3, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge