We narrowed to 21,590 results for: OMP;

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neurod1:cre; cmlc2:EGFP

Plasmid

#173480

Purpose

cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

Depositor

John Rawls

Article

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

Cre

Use

Zebrafish expression

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMBEC6

Plasmid

#187598

Purpose

Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

nCas9-BEC-UGI; cas6f, gfp

Expression

Bacterial

Promoter

trc

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A/T1462A)

Plasmid

#14131

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A/T1462A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A/T1462A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
FAB(mito-LD)

Plasmid

#214425

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP_IRES_tdTomato

Plasmid

#184046

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry GFP

Plasmid

#78535

Purpose

Control plasmid (paired gRNAs against GFP)

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-miniTurbo-L2

Plasmid

#127352

Purpose

gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Plasmid

#137830

Purpose

MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Leucine 71 to Lysine in CTEVp fragment

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHY002_Strep_Avi_hsDCAF15

Plasmid

#174479

Purpose

Expresses DCAF15 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DCAF 15 (DCAF15 Human)

Tags

StrepII-Avi-TEV

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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ELF1_pet28a

Plasmid

#131649

Purpose

Bacterial expression of ETS gene ELF1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF1 (ELF1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2

Plasmid

#108940

Purpose

Mammalian expression of mRIN2

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

Plasmid

#177850

Purpose

Bacterial Expression of AMPK

Depositor

Karsten Melcher

Article

Yan et al Science. 2021 Jul 23;373(6553):413-419.

Insert

AMPK (a1b2g1)

Tags

MBP

Expression

Bacterial

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HC078 (3xHA-Atg13)

Plasmid

#59544

Purpose

Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

Depositor

Daniel Klionsky

Article

Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

Insert

ATG13 (ATG13 Budding Yeast)

Tags

3xHA

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

Plasmid

#221080

Purpose

SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-R16-FZY2-S100N-UGI-Puro

Plasmid

#209644

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100N

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro

Plasmid

#209643

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100C

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-HA3-HPS1

Plasmid

#133930

Purpose

Expresses 3xHA-HPS1 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

Insert

HPS1 (HPS1 Human)

Tags

3xHA tag

Expression

Mammalian

Promoter

CMV I.E.

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

Plasmid

#35511

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

Plasmid

#199354

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-SUMOEu

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(mito-LD)

Plasmid

#214421

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-3XHA-V5

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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