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pCAGEN_N140_eMagBF_V5_NES

Plasmid

#235620

Purpose

Encodes ALIBi N-terminal fragment, residues 1-140, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-140

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C74_Sec61b

Plasmid

#235637

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, Sec61b for targeting ALIBi to endoplasmic reticulum

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF and Sec61b

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_V5_Fbl_eMagAF_C74

Plasmid

#235641

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, Fbl for targeting to nucleolus

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

V5-Fbl-eMagAF

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C168

Plasmid

#235623

Purpose

Encodes ALIBi C-terminal fragment, residues 168-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 168-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C66

Plasmid

#235613

Purpose

Encodes ALIBi C-terminal fragment, residues 66-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 66-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N221_eMagBF_V5_NES

Plasmid

#235626

Purpose

Encodes ALIBi N-terminal fragment, residues 1-221, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-221

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C100

Plasmid

#235619

Purpose

Encodes ALIBi C-terminal fragment, residues 100-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 100-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C257

Plasmid

#235631

Purpose

Encodes ALIBi C-terminal fragment, residues 257-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 257-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_GFP_FTA

Plasmid

#235633

Purpose

Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Tags

FLAG-TEV cleavage site-AviTag and NES-V5

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C78

Plasmid

#235617

Purpose

Encodes ALIBi C-terminal fragment, residues 78-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 78-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C74

Plasmid

#235615

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagBF_N73

Plasmid

#235635

Purpose

Encodes fusion protein of nuclear export sequence, V5, eMagBF (enhanced Magnet, basic, fast), ALIBi N-terminal fragment (residues 1-73) (order reversed)

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

NES-V5-eMagBF

Expression

Mammalian

Mutation

residues 1-73

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N167_eMagBF_V5_NES

Plasmid

#235622

Purpose

Encodes ALIBi N-terminal fragment, residues 1-167, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-167

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N195_eMagBF_V5_NES

Plasmid

#235624

Purpose

Encodes ALIBi N-terminal fragment, residues 1-195, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-195

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE-Airn

Plasmid

#233266

Purpose

CARGO plasmid containing ~90kb dox-inducible Airn transgene

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

Airn (Airn Mouse)

Expression

Mammalian

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mNgn2-x3HA

Plasmid

#233189

Purpose

Retroviral expression of tagged mNgn2x3HA

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mNgn2 (Neurog2 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mNgn2-x3FLAG

Plasmid

#233190

Purpose

Retroviral expression of tagged mNgn2x3FLAG

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mNgn2 (Neurog2 Mouse)

Use

Retroviral

Tags

x3FLAG

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mNgn2-x1HA

Plasmid

#233191

Purpose

Retroviral expression of tagged mNgn2x1HA

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mNgn2 (Neurog2 Mouse)

Use

Retroviral

Tags

x1HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-hfCas13d-pa

Plasmid

#233037

Purpose

To express hfCas13d from a EF1a promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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FAM177A1-Halo

Plasmid

#224591

Purpose

For expression of FAM177A1-Halo in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Tags

HALO

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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