We narrowed to 1,321 results for: HRAS

1. AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry

   Plasmid

   #208837

   Purpose

   Knockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

   Depositor

   Ben Emery

   Article

   Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNK7190

   Plasmid

   #219763

   Purpose

   MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   nnLuz, nnH3H, nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK189

   Plasmid

   #219743

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   D37E, V181I, S323M, M385K

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNK4169

   Plasmid

   #219744

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   D37E, V181I, S323M, M385K

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNK7183

   Plasmid

   #219760

   Purpose

   MoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   PzPKS2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   p35s_0.4kb - 5'UTR TMV omega

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNK7180

   Plasmid

   #219762

   Purpose

   MoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   HmS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   p35s_0.4kb - 5'UTR TMV omega

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGE1.0

   Plasmid

   #165983

   Purpose

   Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Spec resistance.

   Depositor

   Bogumil Karas

   Article

   Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

   Inserts

   RK2/RP4 origin of transfer
   repA2B2C2
   nourseothricin N-acetyl transferase
   spectinomycin/streptomycin resistance
   

   Use

   Synthetic Biology; Bacterial and yeast cloning; c…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBGE2.0

   Plasmid

   #165987

   Purpose

   Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.

   Depositor

   Bogumil Karas

   Article

   Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

   Inserts

   RK2/RP4 origin of transfer
   repA1B1C1
   nourseothricin N-acetyl transferase
   tetracycline resistance
   

   Use

   Synthetic Biology; Bacterial and yeast cloning; c…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGE3.0

   Plasmid

   #165985

   Purpose

   Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Nm/Kan resistance.

   Depositor

   Bogumil Karas

   Article

   Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

   Inserts

   RK2/RP4 origin of transfer
   repA2B2C2
   nourseothricin N-acetyl transferase
   neomycin resistance
   

   Use

   Synthetic Biology; Bacterial and yeast cloning; c…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmirGlo-RBM8A-3'UTR 240-546

    Plasmid

    #158108

    Purpose

    reporter 3'UTR hRBM8A (short)

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    3'UTR of hRBM8A (RBM8A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUMO GLTP

    Plasmid

    #170732

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX026

    Plasmid

    #167153

    Purpose

    MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnLuz-ocsT
    

    Use

    Luciferase

    Tags

    His6-tag

    Expression

    Plant

    Mutation

    Promoter

    p35s

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCardinal-Farnesyl-5

    Plasmid

    #56159

    Purpose

    Localization: Plasma Membrane, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pDONR223-H-RAS V12

    Plasmid

    #31201

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    H-Ras (HRAS Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    G12V constitutively active

    Promoter

    Available Since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCMV_SEPT9_i3-mApple-CAAX

    Plasmid

    #180324

    Purpose

    mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas)

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN9_v3 (SEPTIN9 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid

    #219565

    Purpose

    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK497

    Plasmid

    #219754

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FLAG_FBXL7

    Plasmid

    #171848

    Purpose

    pcDNA3-FLAG_FBXL7

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    F-box and leucine rich repeat protein 7 (FBXL7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-iDreV

    Plasmid

    #140136

    Purpose

    can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iDreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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