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Plasmid#195627PurposeComplementation of MlaA(delta227-251)DepositorInsertMlaA
UseTagsExpressionBacterialMutationMlaA(delta227-251)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2639
Plasmid#195628PurposeComplementation of MlaA(I241N)DepositorInsertMlaA
UseTagsExpressionBacterialMutationMlaA(I241N)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1195
Plasmid#196027PurposeComplementation of MlaF-MlaE-MlaD-MlaC-MlaBDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJo55
Plasmid#159623PurposeAn empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJo55-CA1
Plasmid#159624PurposePlasmid pJo55, which contains positive control (C. annuum eIF4E-1) for complementation test in Jo55 yeasts.DepositorInsertC. annuum eIF4E-1
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
sg1
Plasmid#113966PurposeSingle short guide RNA targeting GTATAGCATACATTATACGDepositorInsertsg1
UseTagsExpressionMutationPromoterU6Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Laconic/pcDNA3.1(-)
Plasmid#44238Purposea genetically-encoded sensor for lactate concentration, transport and metabolic fluxDepositorInsertLaconic (LACtate Optical Nano Indicator from CECs)
UseLactate biosensorTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKPI-MtLAP1
Plasmid#193521PurposeAnthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula rootsDepositorInsertsMtLAP1
DsRed
UseTagsExpressionPlantMutationPromoterATUBI10 and Medtr8g059790(KPI)Available sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dual opposing T7 promoters reporter plasmid
Plasmid#156457PurposeA mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.DepositorInsertNeoR/KanR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7Available sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL1-pKPI-MtLAP1-Adh
Plasmid#193520PurposeAnthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula rootsDepositorInsertMtLAP1
UseTagsExpressionPlantMutationPromoterMedtr8g059790(KPI)Available sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
p CIneo-RL-Let7-3xBulgeB-mut
Plasmid#115369PurposeExpression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
POLArIS_NbVim-cp10GFP
Plasmid#171020PurposeExpresses NbVim-cp10GFP#2 in mammalian cellsDepositorInsertanti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
UseTagscp10GFPExpressionBacterial and MammalianMutationPromoterCMV promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSI-359
Plasmid#131131PurposegRNA expression vector for A-to-G base editing reporterDepositorInsertA-to-G reporter activation gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + rpsL reporter plasmid
Plasmid#156455PurposeA mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsrpsL
TcR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromotertac and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISP_IS
Plasmid#120425PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterconstitutiveAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172668PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertALDOB pegRNA and ALDOB_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR1
Plasmid#15706DepositorInsertp15 4xSBR1 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR2
Plasmid#15709DepositorInsertp15 4xSBR2 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
GluR2_adRNA(2,100,50)-GFP
Plasmid#124622PurposeExpresses adRNA(2,100,50) targeting the RAB7A transcript along with GFPDepositorInsertGluR2_adRNA(2,100,50)
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GluR2_adRNA(0,100,50)-GFP
Plasmid#124623PurposeExpresses adRNA(0,100,50) targeting the RAB7A transcript along with GFPDepositorInsertGluR2_adRNA(0,100,50)
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only