We narrowed to 1,143 results for: fret

1. pcDNA3.1(+)-FluoSTEP-AktAR

   Plasmid

   #181856

   Purpose

   Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AktAR
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

   Plasmid

   #40571

   Depositor

   Daniel Gerlich

   Article

   Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

   Insert

   Aurora B FRET-sensor N-terminally fused to histone 2B
   

   Tags

   CyPet and YPet

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSOMA-T679A-NLS

   Plasmid

   #118938

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
   

   Expression

   Plant

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. tagSrc(WT)-mCer3 S208F/V224L

   Plasmid

   #179138

   Purpose

   mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   Src, SsrA
   

   Tags

   mCerulean3 (S208F/V224L)

   Expression

   Mammalian

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiex PKC - Peptide 8 mCit mCer

   Plasmid

   #83563

   Purpose

   Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PRKCA (PRKCA Human)
   

   Tags

   10 nm ER/K Helix, FLAG purification tag, TEV Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSOMA-T679A-NES

   Plasmid

   #118940

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
   

   Expression

   Plant

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-PKCsub(T/A)-YPet-NES

   Plasmid

   #138208

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKCsub(T/A)-YPet-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Sept. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBiex PKC - Peptide 14 mCit mCer

   Plasmid

   #83565

   Purpose

   Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PKC alpha (PRKCA Human)
   

   Tags

   10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)

   Plasmid

   #181861

   Purpose

   FluoSTEP-AKAR using mScarlet-I as the FRET acceptor.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AKAR (mScarlet-I)
   

   Tags

   mScarlet-I and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid

    #138205

    Purpose

    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub(T/A)-YPet-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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11. nlsSalicS1

    Plasmid

    #232190

    Purpose

    Novel FRET-based biosensor for salicylic acid

    Depositor

    Alexander Jones

    Article

    Tang et al Science. 2025 Oct 9;390(6769):188-194. d

    Insert

    nlsSalicS1
    

    Tags

    His-tag

    Expression

    Plant

    Promoter

    p16

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

    Plasmid

    #101299

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 and 698-906 of alpha-C…

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

    Plasmid

    #234869

    Purpose

    Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Lyso-TORCAR

    Plasmid

    #64929

    Purpose

    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-TORCAR (LAMP1 Human, Synthetic)
    

    Tags

    Cerulean, Full-length LAMP1, and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-TORCAR-NLS

    Plasmid

    #64931

    Purpose

    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR-NLS (EIF4EBP1 Human, Synthetic)
    

    Tags

    Cerulean, Nuclear localization signal (NLS), and …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101297

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-906 of alpha-Catenin

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-PM-TORCAR

    Plasmid

    #64930

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    Lyn-TORCAR (EIF4EBP1 Human, Synthetic)
    

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Lyso-Digda

    Plasmid

    #236102

    Purpose

    Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    Lyso-Digda (LAMP1 Human, Synthetic)
    

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lyn FAK biosensor

    Plasmid

    #78299

    Purpose

    FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Lyn Fak biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

    Plasmid

    #85070

    Purpose

    Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    PH-PLCD1 (PLCD1 Human)
    

    Tags

    IRES-sYFP2-PH and mScarlet-H

    Expression

    Mammalian

    Mutation

    PLCD aa 1–170

    Promoter

    CMV

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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