We narrowed to 1,166 results for: fret

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pcDNA3.1-Cerulean-Epac2(R53A)-Venus

Plasmid

#138197

Purpose

FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

Plasmid

#138198

Purpose

FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and cpV[E172]

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R466A)-Venus

Plasmid

#138199

Purpose

FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(L426W)-Venus

Plasmid

#138200

Purpose

FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains leucine-to-tryptophan substitution at re…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKAsub-YPet-Kras

Plasmid

#138206

Purpose

Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKAsub-YPet-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR

Plasmid

#64927

Purpose

FRET-based biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR (EIF4EBP1 Synthetic, Human)

Tags

Cerulean and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-EKAR4

Plasmid

#205513

Purpose

Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-EKAR4

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSOMA-T679A-NLS

Plasmid

#118938

Purpose

Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal

Expression

Plant

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-T2A-Venus(L68V)

Plasmid

#60494

Purpose

Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2-T2A-mVenus(L68V)

Tags

T2A peptide

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-ICUE3

Plasmid

#61623

Purpose

Plasma membrane-targeted FRET biosensor for monitoring cAMP

Depositor

Jin Zhang

Article

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

Lyn-ICUE3 (RAPGEF3 Human)

Tags

ECFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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FEMP

Plasmid

#191973

Purpose

FRET-based indicator of ER-mitochondria proximity (FEMP)

Depositor

Luca Scorrano

Article

Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

AkapYFP-Tav2a-CFP-sac1

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Cytosolic FAK biosensor

Plasmid

#78300

Purpose

FRET biosensor. Visualization of cytosolic FAK activity in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Cytosolic FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Raichu-454HRasCT

Plasmid

#176029

Purpose

A FRET biosensor for HRas activity

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

Raichu-Ras-HRasCT

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

Plasmid

#85069

Purpose

Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH-PLCD1 (PLCD1 Human)

Tags

IRES-sYFP2-PH and mScarlet

Expression

Mammalian

Mutation

PLCD aa 1–170

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-Eevee-ROCK-NES

Plasmid

#209918

Purpose

A FRET biosensor for ROCK activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Eevee-ROCK-NES

Use

Lentiviral

Tags

ECFP and YPet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I3-NCwt-SYFP2

Plasmid

#189765

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3-NCwt-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-ICUE3-NLS

Plasmid

#138255

Purpose

FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

Depositor

Jin Zhang

Article

Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

Insert

FKBP-ICUE3-NLS

Tags

FKBP, Nuclear localization signal (NLS), cpVenus[…

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-RhoA

Plasmid

#84358

Purpose

fusion protein of RhoA, FRET donor

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-RhoA (RHOA Synthetic, Human)

Tags

mCyRFP1 N terminal fusion to RhoA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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5HT6-YC3.60

Plasmid

#48339

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)

Tags

YC3.60

Expression

Mammalian

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pSOMA-NES

Plasmid

#118939

Purpose

Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal

Expression

Plant

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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