We narrowed to 1,406 results for: tox

1. pDONR223_HyPer7RgDAAO

   Plasmid

   #217746

   Purpose

   Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   HyPer7 D amino acid oxidase
   

   Use

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   Fused DAAO to the C-terminus of HyPer7 using a Gl…

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-FUS_FL_12E

   Plasmid

   #98652

   Purpose

   Expresses MBP-tagged full length phosphomimetic FUS

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   12 S/T to E

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MBP-FUS_LC_WT

   Plasmid

   #98653

   Purpose

   Expresses MBP-tagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLEX307-SARS-CoV-2-3CL

   Plasmid

   #160278

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-N1-EXO1b

   Plasmid

   #111621

   Purpose

   Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cells

   Depositor

   Marco Muzi-Falconi

   Article

   Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

   Insert

   Exonuclease 1b (EXO1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST53-LRRK2-Y1699C

   Plasmid

   #25048

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tyrosine 1699 mutated to Cysteine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. Tol2-BetaActin-SecA5-YFP

   Plasmid

   #105664

   Purpose

   Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

   Depositor

   Qing Deng

   Article

   Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

   Insert

   Secreted Annexin5-EYFP (ANXA5 Human)
   

   Use

   Tol2-gateway

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Beta-Actin

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 426Gal-FUS-YFP

   Plasmid

   #29592

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenSyn2-EGFP-­OMP25C

   Plasmid

   #71427

   Purpose

   Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

   Depositor

   Henri Huttunen

   Article

   Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

   Insert

   OMP25 (172-­‐206) (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 172-206 only

   Promoter

   synapsin

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. MBP-FUS_FL_6E

    Plasmid

    #98655

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    6 S/T to E

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKH3-TRIM28-C65A/C68A

    Plasmid

    #92199

    Purpose

    Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    Tif1b (TRIM28 Synthetic, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Cysteines 65 and 68 to Alanines.

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiRCas9-CUG

    Plasmid

    #104183

    Purpose

    Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

    Depositor

    Eugene Yeo

    Article

    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

    Insert

    U6-CUGsgRNA, EFS-PIN-dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-CAG-F14F15S-Switch

    Plasmid

    #99733

    Purpose

    Flp-based FLEX reporter (from RFP to GFP)

    Depositor

    Ian Wickersham

    Article

    Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

    Insert

    TagRFP and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LT3GEPIR-ARshRNA2

    Plasmid

    #220562

    Purpose

    To inducibly knockdown AR expression

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    AR shRNA2 (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDiCre-mCherry

    Plasmid

    #164572

    Purpose

    Integration of a DiCre cassette in PbGFP parasite genome

    Depositor

    Olivier Silvie

    Article

    Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

    Inserts

    FKBP12-CRE59
    FRB-CRE60
    mCherry
    Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Bidirectional PbEF1alpha promoter (PBANKA_1133300…

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-eEF2K(S441A/S445A)

    Plasmid

    #110161

    Purpose

    Expression of human eEF2K (S441A/S445A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

    Insert

    eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S441A/S445A

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 416Gal-FUS-YFP

    Plasmid

    #29593

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. 416Gal-FUS-P525L-YFP

    Plasmid

    #29628

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    P525L

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pGEX VAMP7 (1-188)

    Plasmid

    #42315

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Δ181-220

    Promoter

    Ptac

    Available since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pLEX307-SARS-CoV-2-3CL-C145A

    Plasmid

    #160279

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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