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LRG-neo-sgTAF12(mouse)#4.1

Plasmid

#105588

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting TAF12 HFD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-puro-N3FLAG-MYB-del(TAD)

Plasmid

#105597

Purpose

retrovirally express mouse MYB-del(TAD) with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB without TAD domain (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

deletion of TAD (AA 275-327)

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-TAF4(827-909)

Plasmid

#105616

Purpose

retrovirally express express mouse TAF4 HFD with GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD-827-909 (Taf4 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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SP65U1-135a-1

Plasmid

#105819

Purpose

plasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmid

Depositor

Cecilia Mannironi

Article

Mannironi et al PLoS One. 2013 Sep 4;8(9):e73385. doi: 1

Insert

MIRNA135A1 (Mir135a-1 Mouse)

Expression

Mammalian

Promoter

U1

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-zfh1_spikeIn-1

Plasmid

#86387

Purpose

Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter

Use

Stap-seq spike in vector

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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NUDC D10.4 gRNA

Plasmid

#90807

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUDC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NUDC (Guide Designation D10.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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NSL1 C6.4 gRNA

Plasmid

#90805

Purpose

3rd generation lentiviral gRNA plasmid targeting human NSL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NSL1 (Guide Designation C6.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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KIF4A D9.2 gRNA

Plasmid

#90724

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF4A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF4A (Guide Designation D9.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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MCM4 F8.1 gRNA

Plasmid

#90761

Purpose

3rd generation lentiviral gRNA plasmid targeting human MCM4

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MCM4 (Guide Designation F8.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-zfh1_spikeIn-3

Plasmid

#86389

Purpose

Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1

Use

Stap-seq spike in vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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