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We narrowed to 400 results for: AGA

Showing: 1 - 20 of 400 results

  1. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...using the agarose cloning method Test your agarose temperature. Cloning cells using an agarose overlay ...to pour the agarose onto the recipient plate at just the right temperature. If the agarose solution is...the plate. We test the (capped) 1:1 agarose/DMEM solution against a part of the body very sensitive to...gene of interest. Advantages of the agarose cloning method The agarose technique should be useful to scientists...containing a low percentage of agarose, which is then overlaid with the firmer 2% agarose gel solution discussed...aerated with a thin layer of molecular biology-grade agarose containing physiological concentrations of salts...visible to the naked eye) with a marker prior to agarose addition, we found it was possible to rapidly punch...

  2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...for generating sybodies against membrane proteins Developing antibodies against proteins embedded in membranes... challenging when trying to develop antibodies against unstable targets like membrane proteins that may...plasmids to generate sybodies, synthetic nanobodies against the spike protein (Walter et al., 2020). No llamas...selection of synthetic nanobodies, called sybodies, against membrane protein targets in as little as 3 weeks...Outline of the process for selection of sybodies against membrane proteins. Image from Zimmermann et al....Seeger MA (2020) Generation of synthetic nanobodies against delicate proteins. Nature Protocols 15:1707–1741...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ...of LB-agar, we’ll need: (37 g pre-mixed LB-agar powder/L) x (0.220 L) = 8.14 g pre-mixed LB-agar powder... Protocols Pouring LB Agar Plates Pouring LB Agar Plates...peptone from casein 10.0 g sodium chloride 12.0 g agar-agar 1 L Sterile H 2 O Sterile plates of your desired...Procedure Measure 37g of pre-mixed LB-agar powder per L of molten agar you’d like to make. The precise mass...autoclaving. We make 400 mL of agar in 1 L bottles and 200 mL of agar in 500 mL bottles. The extra empty volume...Cooled agar should be warm to the touch; as a rule of thumb, if you cannot take the molten agar out of.... Swirl the agar bottle to ensure even distribution of the antibiotic throughout the agar. Open one plate...

  6. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Procedure Pouring a Standard 1% Agarose Gel: Measure 1 g of agarose. Pro-Tip Agarose gels are commonly used in...mass of agarose in a given volume to make gels of other agarose concentrations (e.g., 2 g of agarose in 100... Protocols Agarose Gel Electrophoresis Agarose Gel Electrophoresis You may also ...adjust the agarose percentage of the gel to get better separation. A higher percentage agarose gel will ...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and...Restriction Digest of Plasmid DNA Purifying DNA from an Agarose Gel DNA Ligation Introduction Gel electrophoresis... to move the negatively charged DNA through an agarose gel matrix toward a positive electrode. Shorter...

  7. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Bacteria on LB Agar Plate Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates Bacterial...plating antibiotic over-agar for the selection of transformed E. coli . Over-agar spreading of antibiotic...containing solidified LB-agar, pipette 150 µL of carbenicillin on top of the agar and gently spread over...plated over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using different...prepare separate batches of antibiotic-containing agar. This protocol will focus specifically on selection...video below to learn how to spread antibiotic over-agar. Equipment Pipette tips for both pipetting and spreading...flame source) Incubator Reagents 6 cm diameter LB/agar plates without antibiotic High concentration (100...

  8. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...Protocols Purifying DNA from an Agarose Gel Purifying DNA from an Agarose Gel You may also like... Restriction...Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and...Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis DNA Ligation Background Information Gel purification...based on size. The procedure starts with standard agarose gel electrophoresis, which separates DNA by their...electrophoresis, you can cut DNA bands out of the agarose gel and purify the DNA samples. This is a commonly...cloning. Protocol: Gel Purification Follow the Agarose Gel Electrophoresis Protocol with the following...Gel purification is most efficient with lower % agarose gels, so you will want to stay in the 0.7-0.8% ...

  9. Molecular Biology Reference

    Type
    Guide
    ...GCC, GCA, GCG Arginine Arg R CGU, CGC, CGA, CGG, AGA, AGG Asparagine Asn N AAU, AAC Aspartic Acid Asp ...allow for the propagation of the plasmid within bacteria, while allowing for selection against any bacteria...bacteria with plasmids, thus selecting against the propagation of these plasmids through cell division...lab. Regardless of type, plasmids are generally propagated, selected for, and their integrity verified prior...cloning techniques . Common Bacteria Strains for Propagating Plasmids E. coli are gram-negative, rod-shaped...which antibiotic(s) to add to your LB media or LB agar plates. For more information on growing bacterial...These attached DNA templates are then amplified again, producing ~1,000 copies of each template. Each ...

  10. Validated gRNA Sequences

    Type
    Collection
    ...victoria GGTGGTGCAGATGAACTTCA 47513 cut S. pyogenes 23792628 Joung EGFP A. victoria TGAAGAAGATGGTGCGCTC 58255...sapiens GATACTGGAGAAGCAGGGGC 68466 cut S. pyogenes 26145478 Zhang PAX6 H. sapiens GTAGATTTTGTATGCACTGC 68465...Johnson UCA1 H. sapiens gRNA1:GGTTTCCTTTTAGATGACGG; gRNA2:TCTGAAAAGAGAGTCAGCGA 72628 cut S. pyogenes 26493208...pyogenes 25739462 Jiang ade6-L469 S. pombe TCTATTGTTCAGATGCCTTG 52227 cut S. pyogenes 25352017 Zaratiegui...Zaratiegui ade6-M210 S. pombe TCTATTGTTCAGATGCTTCG 52226 cut S. pyogenes 25352017 Zaratiegui ade6+ S. pombe TCTATTGTTCAGATGCCTCG... TCTATTGTTCAGATGCCTCG 52225 cut S. pyogenes 25352017 Zaratiegui Alk and Eml M. musculus 64071 cut S. pyogenes...pyogenes 26472758 Sabatini ANT1 S. lycopersicum TGTCGTTTATAATTTGTAGA 70019 cut S. pyogenes 26541286 Voytas APC...

  11. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...OPEN OPEN aGACTCCGTCccgtcgtGCTGAGGGCc hnf1a OZ521 and OZ522 OPEN OPEN aACCTTCCCCagagaGAGGTAGTGg VDAC2 OZ523...OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg dpf2 OZ531 and OZ532 OPEN OPEN cTGCTACTTCtggatGGAGAAACGa pitx2 OZ533... CODA tGTCTTCATCTCCTTCAGATGCTGTCc fam82a2 OZ573 and OZ574 CODA CODA gCACCGCTGCAGTAAGAGCTGAGGAAt morc3b...CODA CODA aGTCCGCCTCCACCCGAGGTAGAG zgc:111821 OZ597 and OZ598 CODA CODA tGGCAGCAGCTTTTCGAAGAATAGg zgc:65890...half-site CACNA1F OZ507 and OZ508 OPEN OPEN gCGTGACCTCgctgatAAGAAGAGg xylt1 OZ509 and OZ510 OPEN OPEN cACCCTCTACaggacaGTAGCGGGTg...gACAGCCGTCtgcatctGGAGGTGCCc pitrm1 OZ515 and OZ516 OPEN OPEN cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt...Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc...

  12. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...Prefix: GAATTC GCGGCCGC T TCTAGA G Alternate Prefix: GAATTC GCGGCCGC T TCTAGA TG (contains start) Prefix...CTGCAG Suffix Enzymes: SpeI , NotI , PstI Scar: TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions...– Berkeley Standard (RFC 21) Prefix: GAATTC ATG AGATCT Prefix Enzymes: EcoR1 , BglII Suffix: T GGATCC ...Silver Standard (RFC 23) Prefix: GAATTC GCGGCCGC T TCTAGA G Prefix Enzymes: EcoR1 , NotI , XbaI Suffix: T...CTGCAG Suffix Enzymes: SpeI , NotI , PstI Scar: ACTAGA Features: Modified RFC 10 with shortened prefix...Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA TG GCCGGC Alternate Prefix: GAATTC GCGGCCGC T TCTAG...

  13. Antibody Guide

    Type
    Collection
    ...which is immobilized on agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (...developed, the antibodies are collected, tested against the antigen, packaged, and sold. This is the most...unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving allergic... directly to the sdAb, or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs...materials. There are two primary methods used, using agarose or magnetic beads, but both rely on the same general...most or all of their extracellular matrix removed. Again, the signaling molecule may be a fluorophore or ...or more conjugated primary antibodies, and wash again. Use controls to identify, or gate, cells by size...

  14. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...RAB7 GFP Richard Pagano 12661 DsRed-rab7 WT Late endosomes RAB7 DsRed Richard Pagano 158006 pCMV-mGold-Lysosome-N... GFP Richard Pagano 12679 DsRed-rab11 WT Recycling endosomes Rab11a DsRed Richard Pagano *Fusions to other...DsRed-Rab5 WT Early endosomes RAB5A DsRed2 Richard Pagano 42307 GFP-EEA1 wt Early endosomes EEA1 GFP Silvia...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving to be...genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists...Horvath P. 2007. CRISPR provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide...

  16. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins...Toolbox for making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain...May 24;7. and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus...

  17. Arf GTPase Family

    Type
    Collection
    ...116983 834 GAP Agap1 (CENTG2) 116987 857 GAP Agap2 (GGAP2) 116986 1192 Bacterial (pET) GAP Agap3 116988 911...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial..., Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, ... CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean...ACS Chem Biol. 2010 Feb 19;5(2):215-22. Takeharu Nagai ATP ATP sensor for Yeast (AT1.03 derived) Dynamic...polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-...Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring...
Showing: 1 - 20 of 400 results