Addgene

We narrowed to 10,134 results for: AGA

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pET28a-DONSON

Plasmid

#208797

Purpose

Xenopus laevis His6-Myc-DONSON wild type

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1-Cdc45

Plasmid

#208796

Purpose

Xenopus laevis FLAG5-His10-TEV-Cdc45

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

Cdc45 (cdc45.L Frog)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-WM

Plasmid

#208798

Purpose

Xenopus laevis His6-Myc-DONSON (W234L, M463T)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

W234L; M463T

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEWS

Plasmid

#208799

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A; S437A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-3A

Plasmid

#208800

Purpose

Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

V473A; R476A; Y481A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-Δ24

Plasmid

#208801

Purpose

Xenopus laevis His6-Myc-DONSON (1-24aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Δ24, deletion of 1-24aa

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEW

Plasmid

#208802

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-S437A

Plasmid

#208803

Purpose

Xenopus laevis His6-Myc-DONSON (S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

S437A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-ΔD1

Plasmid

#208804

Purpose

Xenopus laevis His6-DONSON (1-155aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His

Expression

Bacterial

Mutation

ΔD1, deletion of 1-155aa

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG

Plasmid

#127327

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG

Plasmid

#127326

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

Plasmid

#199761

Purpose

Elongation reporter construct to quantify elongation duration of 6_AGA stalling

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

synYFP[TTG/AGA]-6xArg[AGA]-cLuc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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IV-AGA

Plasmid

#43739

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

IV-AGA

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
CellTagA

Plasmid

#124591

Purpose

Expressed barcode for sample multiplexing in single-cell analysis

Depositor

Samantha Morris

Article

Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

Insert

CellTagA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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ALPaGA

Plasmid

#175272

Purpose

Lactate biosensor in anaerobic conditions

Depositor

Jerome Bonnet

Article

Zuniga et al ACS Synth Biol. 2021 Dec 1. doi: 10.1021

Insert

LldR_Synthetic_promoter_sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MS-1 magA

Plasmid

#21751

Depositor

Elliot Meyerowitz

Article

Bertani et al Gene. 2001 Feb 21. 264(2):257-63.

Insert

magA (magA M. magnetotacticum)

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2009

Availability

Academic Institutions and Nonprofits only
AgAK

Plasmid

#71290

Purpose

Expression of A. gambiae adenosine kinase

Depositor

Vern Schramm

Article

Cassera et al Biochemistry. 2011 Mar 22;50(11):1885-93

Insert

Adenosine Kinase

Use

Tags

6X Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pCAGEN_C74_eMagAF_V5_NES

Plasmid

#235636

Purpose

Encodes fusion protein of ALIBi C-terminal fragment (residues 74-321), eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence (order reversed)

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Use

Tags

eMagAF-V5-NES

Expression

Mammalian

Mutation

residues 74-321

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C233

Plasmid

#235629

Purpose

Encodes ALIBi C-terminal fragment, residues 233-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Use

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 233-321

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C141

Plasmid

#235621

Purpose

Encodes ALIBi C-terminal fragment, residues 141-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Use

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 141-321

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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