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We narrowed to 30 results for: AGA

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Showing: 1 - 20 of 30 results

  1. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...OPEN OPEN aGACTCCGTCccgtcgtGCTGAGGGCc hnf1a OZ521 and OZ522 OPEN OPEN aACCTTCCCCagagaGAGGTAGTGg VDAC2 OZ523...OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg dpf2 OZ531 and OZ532 OPEN OPEN cTGCTACTTCtggatGGAGAAACGa pitx2 OZ533... CODA tGTCTTCATCTCCTTCAGATGCTGTCc fam82a2 OZ573 and OZ574 CODA CODA gCACCGCTGCAGTAAGAGCTGAGGAAt morc3b...CODA CODA aGTCCGCCTCCACCCGAGGTAGAG zgc:111821 OZ597 and OZ598 CODA CODA tGGCAGCAGCTTTTCGAAGAATAGg zgc:65890...half-site CACNA1F OZ507 and OZ508 OPEN OPEN gCGTGACCTCgctgatAAGAAGAGg xylt1 OZ509 and OZ510 OPEN OPEN cACCCTCTACaggacaGTAGCGGGTg...gACAGCCGTCtgcatctGGAGGTGCCc pitrm1 OZ515 and OZ516 OPEN OPEN cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt...Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc...

  2. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...Prefix: GAATTC GCGGCCGC T TCTAGA G Alternate Prefix: GAATTC GCGGCCGC T TCTAGA TG (contains start) Prefix...CTGCAG Suffix Enzymes: SpeI , NotI , PstI Scar: TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions...– Berkeley Standard (RFC 21) Prefix: GAATTC ATG AGATCT Prefix Enzymes: EcoR1 , BglII Suffix: T GGATCC ...Silver Standard (RFC 23) Prefix: GAATTC GCGGCCGC T TCTAGA G Prefix Enzymes: EcoR1 , NotI , XbaI Suffix: T...CTGCAG Suffix Enzymes: SpeI , NotI , PstI Scar: ACTAGA Features: Modified RFC 10 with shortened prefix...Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA TG GCCGGC Alternate Prefix: GAATTC GCGGCCGC T TCTAG...

  3. Antibody Guide

    Type
    Collection
    ...which is immobilized on agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (...developed, the antibodies are collected, tested against the antigen, packaged, and sold. This is the most...unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving allergic... directly to the sdAb, or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs...materials. There are two primary methods used, using agarose or magnetic beads, but both rely on the same general...most or all of their extracellular matrix removed. Again, the signaling molecule may be a fluorophore or ...or more conjugated primary antibodies, and wash again. Use controls to identify, or gate, cells by size...

  4. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...RAB7 GFP Richard Pagano 12661 DsRed-rab7 WT Endosomes (late) RAB7 DsRed Richard Pagano 61185 pCMU-LEr Endosomes...EGFP Richard Pagano 12679 DsRed-rab11 WT Endosomes (recycling) Rab11a DsRed Richard Pagano *Fusions to ...-Rab5 WT Endosomes (early) RAB5A DsRed2 Richard Pagano 42307 GFP-EEA1 wt Endosomes (early) EEA1 EGFP Silvia...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving to be...genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists...Horvath P. 2007. CRISPR provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide...

  6. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins...Toolbox for making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain...May 24;7. and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus...

  7. Arf GTPase Family

    Type
    Collection
    ...116983 834 GAP Agap1 (CENTG2) 116987 857 GAP Agap2 (GGAP2) 116986 1192 Bacterial (pET) GAP Agap3 116988 911...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial..., Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, ... CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg 2+ sensor, based on Cerulean...ACS Chem Biol. 2010 Feb 19;5(2):215-22. Takeharu Nagai ATP ATP sensor for Yeast (AT1.03 derived) Dynamic...polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-...Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring...

  11. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral plasmids to produce viral vectors...research typically do not have the ability to propagate in the same way that wild-type viruses do. For...

  12. COVID-19 Resources

    Type
    Collection
    ...Interaction Network Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein...DETECTR No Llamas Required - Synthetic Nanobodies Against Membrane Proteins Return to top Pooled Libraries...

  13. CRISPR Guide

    Type
    Collection
    ...systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family members...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s..., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., ...: 27984730 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome ...

  14. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...site is widely used. Flp FRT GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC 13 bp inverted repeats + 8... F3, F5, FRT14, FRT15 minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...easily identify your protein using an antibody against that epitope. This could allow you to conduct...favorite gene even if you do not have an antibody against it. Another common scenario is fusing your...

  18. CRISPR Plasmids - Parasites

    Type
    Collection
    ...knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes. To find emtpy...

  20. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein...
Showing: 1 - 20 of 30 results