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Showing: 1 - 20 of 33 results

  1. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ... aak1 TAL3208 & TAL3209 TCTTTGACTCCCGGAGAGagctggtgggctccgggCCAGGTTCTGGAGGGGGA abcg2a TAL3400 & TAL3401...ahcyl1 TAL3018 & TAL3019 TGACAGACTGTGGCGAGGatgccaagctggaggcGACGAAACAGGCCAGCAA amh TAL3210 & TAL3211 TCCAGGCAAGATTTGGGCtgatgctgatgatgacCGTGGCGATTGGGTCGTA...TGATCTGGATCCTAACAAggatgacttgcccctgtGTGCGAACACCAACCATA arf6 TAL3212 & TAL3213 TGGGGAAGATGCTTTCCAagatcttcggcaacaaGGAGATGAGAATATTGA arnt1 TAL3214 & TAL3215 TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA...apoa4-like TAL3023 & TAL3024 TGAAGGTTCTTGTGGTGCtcacacttgctgtggtTACAGGTAAGAAACTAAA apoeb TAL3402 & TAL3403 ...TAL3403 TGCCAGGCTCGTAGCCTGttccaggctgatgccccTCAGCCCAGATGGGAGGA arap3a TAL3026 & TAL3027 TGGCAACAATCGGTCCGTgctcagatgcagaggaCCTGTTGGCTTCCATCCA...ARSA TAL 3002 & TAL 3003 TCAAGAGGCGGTCTCCCTctcaacgagaccaccaTAGCCGAGGTGCTGAAGA asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA... TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA atf6 TAL3406 & TAL3407 TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA...

  2. Validated gRNA Sequences

    Type
    Collection
    ...victoria GGTGGTGCAGATGAACTTCA 47513 cut S. pyogenes 23792628 Joung EGFP A. victoria TGAAGAAGATGGTGCGCTC 58255...sapiens GATACTGGAGAAGCAGGGGC 68466 cut S. pyogenes 26145478 Zhang PAX6 H. sapiens GTAGATTTTGTATGCACTGC 68465...Johnson UCA1 H. sapiens gRNA1:GGTTTCCTTTTAGATGACGG; gRNA2:TCTGAAAAGAGAGTCAGCGA 72628 cut S. pyogenes 26493208...pyogenes 25739462 Jiang ade6-L469 S. pombe TCTATTGTTCAGATGCCTTG 52227 cut S. pyogenes 25352017 Zaratiegui...Zaratiegui ade6-M210 S. pombe TCTATTGTTCAGATGCTTCG 52226 cut S. pyogenes 25352017 Zaratiegui ade6+ S. pombe TCTATTGTTCAGATGCCTCG... TCTATTGTTCAGATGCCTCG 52225 cut S. pyogenes 25352017 Zaratiegui Alk and Eml M. musculus 64071 cut S. pyogenes...pyogenes 26472758 Sabatini ANT1 S. lycopersicum TGTCGTTTATAATTTGTAGA 70019 cut S. pyogenes 26541286 Voytas APC...

  3. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...OPEN OPEN aGACTCCGTCccgtcgtGCTGAGGGCc hnf1a OZ521 and OZ522 OPEN OPEN aACCTTCCCCagagaGAGGTAGTGg VDAC2 OZ523...OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg dpf2 OZ531 and OZ532 OPEN OPEN cTGCTACTTCtggatGGAGAAACGa pitx2 OZ533... CODA tGTCTTCATCTCCTTCAGATGCTGTCc fam82a2 OZ573 and OZ574 CODA CODA gCACCGCTGCAGTAAGAGCTGAGGAAt morc3b...CODA CODA aGTCCGCCTCCACCCGAGGTAGAG zgc:111821 OZ597 and OZ598 CODA CODA tGGCAGCAGCTTTTCGAAGAATAGg zgc:65890...half-site CACNA1F OZ507 and OZ508 OPEN OPEN gCGTGACCTCgctgatAAGAAGAGg xylt1 OZ509 and OZ510 OPEN OPEN cACCCTCTACaggacaGTAGCGGGTg...gACAGCCGTCtgcatctGGAGGTGCCc pitrm1 OZ515 and OZ516 OPEN OPEN cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt...Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc...

  4. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...Prefix: GAATTC GCGGCCGC T TCTAGA G Alternate Prefix: GAATTC GCGGCCGC T TCTAGA TG (contains start) Prefix...CTGCAG Suffix Enzymes: SpeI , NotI , PstI Scar: TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions...– Berkeley Standard (RFC 21) Prefix: GAATTC ATG AGATCT Prefix Enzymes: EcoR1 , BglII Suffix: T GGATCC ...Silver Standard (RFC 23) Prefix: GAATTC GCGGCCGC T TCTAGA G Prefix Enzymes: EcoR1 , NotI , XbaI Suffix: T...CTGCAG Suffix Enzymes: SpeI , NotI , PstI Scar: ACTAGA Features: Modified RFC 10 with shortened prefix...Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA TG GCCGGC Alternate Prefix: GAATTC GCGGCCGC T TCTAG...

  5. Antibody Guide

    Type
    Collection
    ...which is immobilized on agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (...developed, the antibodies are collected, tested against the antigen, packaged, and sold. This is the most...unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving allergic... directly to the sdAb, or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs...materials. There are two primary methods used, using agarose or magnetic beads, but both rely on the same general...most or all of their extracellular matrix removed. Again, the signaling molecule may be a fluorophore or ...or more conjugated primary antibodies, and wash again. Use controls to identify, or gate, cells by size...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...the “deletion band” may still amplify), resolve agarose gel to determine if size is consistent with the....2.1 - 3.2.2 ). Plate onto a lysogeny broth (LB) agar plate with 100 μg/ml ampicillin and incubate O/N...testing the bulk sorted cells. Run samples on 2% agarose gel at 10 V/cm using 1x Tris-acetate-EDTA (TAE)...non-deletion amplicons be easily resolved on an agarose gel for multiplexing. Screening CRISPR/Cas9 Clones...plasmid into DH5α E. coli cells and plate onto LB agar plates with the relevant antibiotic. Select multiple...protein, perform an immunoblot using an antibody against the relevant protein 9 . References This protocol...CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nature Protocols . 2013....

  7. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...RAB7 GFP Richard Pagano 12661 DsRed-rab7 WT Late endosomes RAB7 DsRed Richard Pagano 158006 pCMV-mGold-Lysosome-N... GFP Richard Pagano 12679 DsRed-rab11 WT Recycling endosomes Rab11a DsRed Richard Pagano *Fusions to other...DsRed-Rab5 WT Early endosomes RAB5A DsRed2 Richard Pagano 42307 GFP-EEA1 wt Early endosomes EEA1 GFP Silvia...

  8. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...itself, will protect the HR template from the Cas9. Again, if it's within coding region make sure it's a silent...Forward: CCATCCCCTTCTGTGAATGT EMX1-Reverse: GGAGATTGGAGACACGGAGA Why does the PX260 plasmid use 30nt oligos...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving to be...genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists...Horvath P. 2007. CRISPR provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide...

  10. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins...Toolbox for making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain...May 24;7. and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus...

  11. Arf GTPase Family

    Type
    Collection
    ...Acap3 116983 834 GAP Agap1 116987 857 GAP Agap2 116986 1192 Bacterial (pET) GAP Agap3 116988 911 Bacterial...

  12. Plan Your Experiment

    Type
    Collection
    ...that cleaves DNA heteroduplexes, and run on an agarose gel to identify DNA fragments. PCR and restriction...the appropriate restriction enzyme and run on an agarose gel to identify DNA fragments. PCR amplification...insertions only). The PCR product is then run on an agarose gel to determine whether the edit was successful...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial..., Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, ... CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(...

  15. Viral Vectors

    Type
    Collection
    ... While plasmids are typically used to encode, propagate, and manipulate genetic information, viruses are...research typically do not have the ability to propagate in the same way that naturally occurring viruses...

  16. CRISPR Guide

    Type
    Collection
    ...systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family members...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s..., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., ...: 27984730 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome ...

  17. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...easily identify your protein using an antibody against that epitope. This could allow you to conduct...favorite gene even if you do not have an antibody against it. Another common scenario is fusing your...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean...polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-...Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring...
Showing: 1 - 20 of 33 results