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pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro

Plasmid

#209642

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-FZY2-S100N

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-L17-FZY2-S100C-UGI-Puro

Plasmid

#209641

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-FZY2-S100C

Expression

Mammalian

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBGPa-CMV-GFP-OSBP PH domain

Plasmid

#58724

Purpose

Expresses GFP-Tagged OSBP PH domain in mammalian cells

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Tags

GFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-Nucleocapsid

Plasmid

#168967

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-Nucleocapsid (N )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP3

Plasmid

#79875

Purpose

Bacillus subtilis sgRNA expression vector; integrates into thrC

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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809 pcDNA3 GFP PTEN

Plasmid

#10759

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 IKKE K38A flag

Plasmid

#26202

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase epsilon K38A (IKBKE Human)

Tags

Flag

Expression

Mammalian

Mutation

Catalytic Lysine changed to Alanine (K38A)

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-V0D1

Plasmid

#210348

Purpose

Expresses ATP6V0D1 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

V-ATPase V0D1 (ATP6V0D1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAB1678 CMV-HIVNES-GS-Cas13bt1

Plasmid

#176316

Purpose

CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt1

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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hRAD18-EGFP

Plasmid

#68824

Purpose

Expresses human RAD18 tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 tagged with EGFP (RAD18 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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ELF1_pet28a

Plasmid

#131649

Purpose

Bacterial expression of ETS gene ELF1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF1 (ELF1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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T7-Elongin C-pcDNA3

Plasmid

#19998

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

Elongin C (ELOC Human)

Tags

T7-tag

Expression

Mammalian

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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neurod1:cre; cmlc2:EGFP

Plasmid

#173480

Purpose

cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

Depositor

John Rawls

Article

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

Cre

Use

Zebrafish expression

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry GFP

Plasmid

#78535

Purpose

Control plasmid (paired gRNAs against GFP)

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMK1253

Plasmid

#133058

Purpose

Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mClover2

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-R16-FZY2-S100N-UGI-Puro

Plasmid

#209644

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100N

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro

Plasmid

#209643

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100C

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp1

Plasmid

#168816

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp1 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RPA2

Plasmid

#22893

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

RPA2 (RPA2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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