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pcDNA3-Casp9 C287A

Plasmid

#11819

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 catalytically incompetent mutant (CASP9 Human)

Expression

Mammalian

Mutation

C287A, catalytically incompetent mutant.

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pXPR_502_sgCD4

Plasmid

#158704

Purpose

CD4 CRISPRa control

Depositor

John Doench, David Root

Article

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD4 (CD4 Human)

Use

Lentiviral

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-sgRNA expression vector

Plasmid

#51132

Purpose

For in vitro transcription of sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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G10 Cas9 MDC123

Plasmid

#59187

Purpose

G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Expression

Plant

Promoter

G10

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop A

Plasmid

#171779

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop A

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Val2…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA MOV10 K530A

Plasmid

#51716

Purpose

plasmid expresses FLAG/HA-tagged MOV10 K530A

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 K530A (MOV10 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

K530A

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA MOV10 D645N

Plasmid

#51717

Purpose

plasmid expresses FLAG/HA-tagged MOV10 D645N

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 D645N (MOV10 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

D645N

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-ER Short P2A

Plasmid

#164114

Purpose

Detect the long Peroxisomes-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP Po-ER Short P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-FLEX-BFP2-(mCherry)’

Plasmid

#115234

Purpose

Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

mTagBFP2 and mCherry

Expression

Mammalian

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-SHH

Plasmid

#134350

Purpose

Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

Depositor

Lorenz Studer

Article

Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

Insert

SHH (SHH Human)

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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2xCBX7CD-IFP2.0C

Plasmid

#215754

Purpose

Expresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xCBX7CD-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pT22-GluCNZ-6His

Plasmid

#213504

Purpose

Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

Depositor

John Denu

Article

Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

Insert

Glucanase (glucan endo-1,3-beta-glucosidase)

Tags

6His tag

Expression

Bacterial

Mutation

1-36 Signal Sequence Deletion

Promoter

T7

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGDP1 NDM-1

Plasmid

#112883

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

NDM-1 (blaNDM-1 Klebsiella pneumoniae)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-Hygromycin-TTF-1

Plasmid

#119173

Purpose

Retroviral mammalian expression vector containing wt-human TTF-1 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-ATF6

Plasmid

#32955

Purpose

For visualization of GFP-ATF6 in mammalian cells.

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

M67L and M313I

Promoter

CMV

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA

Plasmid

#235678

Purpose

Plasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆C

Depositor

Simon Powell

Article

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-RFPKDEL

Plasmid

#138660

Purpose

Reporter plasmid expressing the ER-located RFP

Depositor

Bertrand Collet

Article

Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

Insert

RFP-KDEL

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xHA-Reptin

Plasmid

#51636

Purpose

Mamamlian expression of 3xHA tagged reptin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-LiMETER-v2

Plasmid

#113934

Purpose

Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

Depositor

Yubin Zhou

Article

He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

Insert

LOV2 and Stim1 polybasic domain (PB)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti c-MYC-DDK-Puro GFP

Plasmid

#123299

Purpose

Mammalian expression of GFP C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

GFP

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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