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pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

Plasmid

#115795

Purpose

Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrIIA4

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 WT

Plasmid

#61735

Purpose

Expresses WT human NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

None

Expression

Mammalian

Mutation

wild type

Promoter

EF1a

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB2a

Plasmid

#19550

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2a

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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NbV5-Rluc8

Plasmid

#201480

Purpose

BRET detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

Rluc8-NbV5

Tags

Rluc8

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g1

Plasmid

#80784

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pOTIII.EFGP.IGR3.6

Plasmid

#40866

Depositor

Harold Gainer

Article

Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

Insert

MOUSE OXYTOCIN GENE (Oxt Mouse)

Expression

Bacterial

Promoter

mouse oxytocin promoter

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Oct4

Plasmid

#19766

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g4

Plasmid

#80787

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA

Plasmid

#108549

Purpose

Bacterial Target-AID vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET19b-rDAD2 cPGFP(G164)

Plasmid

#165994

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rDAD2 cPGFP(G164)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-miniTurboID-EGFP

Plasmid

#163847

Purpose

In vitro transcription of miniTurboID-EGFP. Parton lab clone JRT

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

In vitro transcription of rna

Tags

EGFP

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-FW/AA

Plasmid

#12289

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Mutation

F285A/W286A

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Expression

Plant

Mutation

D156R

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pKISox1-P2A-eGFP-Hygro

Plasmid

#115685

Purpose

Knock-in of P2A-eGFP at the mouse Sox1 gene

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

P2A-eGFP-loxP-PGK-Hygro-loxP

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Expression

Bacterial

Mutation

see manuscript

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221 Spinophilin

Plasmid

#87123

Purpose

Gateway donor vector for Spinophilin

Depositor

Anthony Baucum

Article

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Insert

Spinophilin (PPP1R9B Human)

Use

Donor vector

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBI-p53 wt/EGFP

Plasmid

#16543

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Expression

Mammalian

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PsChR2_EYFP

Plasmid

#69057

Purpose

High-efficiency blue-shifted cation channelrhodopsin

Depositor

John Spudich

Article

Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

Insert

PsChR2

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g2

Plasmid

#80785

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

Plasmid

#19326

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::twk-18(cn110)cDNA

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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