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pnEA-NpM-Dmchim4EBP-GB1_AA

Plasmid

#148262

Purpose

Bacterial Expression of Dmchim4EBP

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dmchim4EBP

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 sgZER1-1

Plasmid

#191549

Purpose

Expression of spCas9 and sgRNA targetting ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

spCas9 (ZER1 Human)

Use

Lentiviral

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 sgZER1-2

Plasmid

#191550

Purpose

Expression of spCas9 and sgRNA targetting ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

spCas9 (ZER1 Human)

Use

Lentiviral

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1037

Plasmid

#169616

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1108_Tier3(Lenti_inverse)

Plasmid

#169663

Purpose

Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction in inverted direction with polyA (PCMV-5'LTR-Psi-gag-env-A3-pA::A2-pA::A1-pA-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1035

Plasmid

#169614

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1032

Plasmid

#169612

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1031

Plasmid

#169611

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1033

Plasmid

#169613

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1036

Plasmid

#169615

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0U6K8E5

Plasmid

#163266

Purpose

Inducible expression of UniProt identifier A0A0U6K8E5

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0U6K8E5

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0U5XJG3

Plasmid

#163238

Purpose

Inducible expression of UniProt identifier A0A0U5XJG3

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0U5XJG3

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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PROM5_Ntprrn_rev_chlo

Plasmid

#163952

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_Ntpsba_chlo

Plasmid

#163953

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA4/TO-ORF68 (tagless)

Plasmid

#166025

Purpose

Expresses ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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TOPO-BC39

Plasmid

#84433

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC39

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only
TOPO-BC40

Plasmid

#84434

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC40

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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TOPO-BC41

Plasmid

#84435

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC41

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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TOPO-BC42

Plasmid

#84436

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC42

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO-BC44

Plasmid

#84438

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC44

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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