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pTRIP-SFFV-tagBFP-2A-ICP0

Plasmid

#235535

Purpose

To express tagBFP-2A-ICP0

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046828

Plasmid

#145742

Purpose

Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCIG-VP16 RARa [TJ#389]

Plasmid

#16287

Depositor

Thomas Jessell

Article

Novitch et al Neuron. 2003 Sep 25. 40(1):81-95.

Insert

RAR alpha (RARA Human)

Tags

VP16

Expression

Mammalian

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pBK1289-AAV-dCjCas9

Plasmid

#223138

Purpose

AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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RIP Cre-ER (DM#265)

Plasmid

#15029

Depositor

Douglas Melton

Article

Dor et al Nature. 2004 May 6. 429(6987):41-6.

Insert

Rat insulin promoter (RIP) (Ins2 Rat)

Use

Cre/Lox; For making transgenic constructs

Tags

Cre-ER (TM)

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-StrepHA-SUMO2

Plasmid

#66867

Purpose

Mammalian expression of SUMO2 with a strep and an HA tag

Depositor

Ying Liu

Article

Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

Insert

SUMO2 (SUMO2 Human)

Tags

HA and Strep

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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REEP5-EGFP

Plasmid

#210363

Purpose

Expresses REEP5 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT PUR mGSK3AWT

Plasmid

#35159

Depositor

Jim Woodgett

Article

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pLenti c-MYC-DDK-Puro GFP

Plasmid

#123299

Purpose

Mammalian expression of GFP C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

GFP

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDV24_AAV2_DART-VADAR_iRFP720-sensor

Plasmid

#200411

Purpose

AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

DART VADAR sensor

Use

AAV and Synthetic Biology

Tags

MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP30_MARV

Plasmid

#135472

Purpose

Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

VP30

Expression

Mammalian

Mutation

None

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b SARS-CoV PLpro

Plasmid

#61693

Purpose

inducible expression of SARS PLpro protease

Depositor

Michael Johnson

Article

Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

Insert

SARS-CoV PLpro

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pQE60 Q58L wgMDH

Plasmid

#204246

Purpose

Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

Q58L

Promoter

T5

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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C3 luc (T1 del)

Plasmid

#11358

Depositor

Donald McDonnell

Article

Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

Insert

C3 promoter (C3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133

Plasmid

#87340

Purpose

To express human Nup133 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of Nup133 AA1-7

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEY14

Plasmid

#191059

Purpose

acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-5(orf) (acr-5 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-fRCaMP1

Plasmid

#125249

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMp1

Tags

NES signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-WT

Plasmid

#177614

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

Plasmid

#199360

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOEu-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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GB-Dcp1b

Plasmid

#153979

Purpose

Dimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1b

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1b (DCP1B Human)

Tags

pAcGFP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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