We narrowed to 10,569 results for: fus
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Plasmid#227619PurposeOverexpression of SLC25A46 lacking its N-terminal region in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
TagsP2A-mElectra1ExpressionMammalianMutationdeleted aa 2-83PromoterCMV, T7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-CC
Plasmid#225476PurposeExpress mEGFP-GAPDH-CC fusionDepositorInsertGAPDH fused with CC2 domain of ROCK2 (GAPDH Human)
TagsmEGFPExpressionMammalianPromoterCMVAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1559
Plasmid#218590PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimerDepositorInsertGES
TagsePTS1ExpressionYeastMutationErg20(F96W, N127W), Erg20-Erg20 synthetic homodim…Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K5Q-GS3
Plasmid#204746PurposeExpression of Cas9 and human H2AX with the K5Q mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K5R-GS3
Plasmid#204747PurposeExpression of Cas9 and human H2AX with the K5R mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134L-GS3
Plasmid#204750PurposeExpression of Cas9 and human H2AX with the K134L mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134M-GS3
Plasmid#204751PurposeExpression of Cas9 and human H2AX with the K134M mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134A-GS3
Plasmid#204752PurposeExpression of Cas9 and human H2AX with the K134A mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-SF14P
Plasmid#209448Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyPromoterPtipA; sgRNA expression from SF14P promoterAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-kasOP*
Plasmid#209447Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyPromoterPtipA; sgRNA expression from kasOP* promoterAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421)
Plasmid#213619PurposeBacterial expression of SUMO-tagged rat syt1 (96-421)DepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2(1-196)-Venus
Plasmid#209182PurposeMammalian expression of human NKX6-2 cDNA SPAX8-related truncated mutation containing the amino acids 1 to 196; fused with the green fluorescent protein Venus allowing the use of fluorescent based protocols.DepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2
Plasmid#185346PurposeMembrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2DepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
ExpressionMammalianAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIN3_enpp-1_mScarlet
Plasmid#182934PurposeExpresses enpp-1 (C27A7.1) under its native promoter (male reproductive tract and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)DepositorInsertgenomic enpp-1 fused to mScarlet
Tagsflexible linker (GGGGS)x3 and mScarlet tagExpressionWormPromoterenpp-1Available SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB556
Plasmid#185108Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160ADepositorInsertPOM152
TagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domai…Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB553
Plasmid#185107Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182LDepositorInsertPOM152
TagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domai…Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIN2_mcm-3_mScarlet
Plasmid#182933PurposeExpresses mcm-3 under its native promoter (embryonic nuclei and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)DepositorInsertgenomic mcm-3 fused to mScarlet
Tagsflexible linker (GGGGS)x3 and mScarlet tagExpressionWormPromotermcm-3Available SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF678
Plasmid#88761PurposeDonor Vector containing ZNF678 transcription factor, part of the Human TFome CollectionDepositorInsertZNF678 (ZNF678 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF751
Plasmid#88377PurposeDonor Vector containing ZNF751 transcription factor, part of the Human TFome CollectionDepositorInsertZNF751 (ZFP2 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF658
Plasmid#88446PurposeDonor Vector containing ZNF658 transcription factor, part of the Human TFome CollectionDepositorInsertZNF658 (ZNF658 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only