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PB-U6-sgRNA-BFP-Puro

Plasmid

#229013

Purpose

Perturb Seq sgRNA vector with PiggyBac backbone

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Type

Empty backbone

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63YA-SBP-EGFP

Plasmid

#222327

Purpose

Synchronize the trafficking of CD63YA from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD63-Y235A fused to SBP-EGFP (CD63 Human)

Expression

Mammalian

Mutation

Tyr235 to Ala

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_puro-mKate-lox5171

Plasmid

#162077

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.12

Plasmid

#155033

Purpose

Fluorescent reporter for ATF4 translation, Negative Control

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 start codon mutated

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pIGAhRC

Plasmid

#112510

Purpose

human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

Depositor

Charles Miller

Article

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

HIF1B, AhR (ARNT, AHR Budding Yeast, Human)

Use

Synthetic Biology

Expression

Yeast

Promoter

Gal1,10

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF2_WT

Plasmid

#82239

Purpose

Gateway Donor vector containing TRAF2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF2 (TRAF2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mCitrine-DNAJC5 L115R

Plasmid

#246735

Purpose

Mammalian expression of human DNAJC5 L115R with a mCitrine (YFP) tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

mCitrine

Expression

Mammalian

Mutation

L115R

Promoter

CMV

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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mCitrine-DNAJC5

Plasmid

#246733

Purpose

Mammalian expression of human DNAJC5 wildtype with a mCitrine (YFP) tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_hygro-tagBFP-lox2272

Plasmid

#162078

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD9-SBP-EGFP

Plasmid

#222328

Purpose

Synchronize the trafficking of CD9 from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD9 fused to SBP-EGFP (CD9 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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