We narrowed to 20,937 results for: Cre

Showing: 9941 - 9960 of 20937 results

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LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode

Plasmid

#170249

Purpose

Barcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

RALA (RALA Human)

Use

Lentiviral

Mutation

G23V

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_L139*

Plasmid

#174400

Purpose

S. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

L139* (Truncation after 139th amino acid in sarA)

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_S130A

Plasmid

#174399

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAP

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

S130A

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_Y167F

Plasmid

#174398

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

Y167F

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585-gp130

Plasmid

#174393

Purpose

Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA-IL6ST chimera

Tags

Flag

Expression

Mammalian

Mutation

140-177 of sarA replaced with homologous IL6ST se…

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585_Y167F

Plasmid

#174392

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Tags

Flag

Expression

Mammalian

Mutation

Tyr 167 changed to Phe

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-SQSfl

Plasmid

#171010

Purpose

HiLITR protease with full-length SQS targeting information (ER)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-SQS(FL)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgfr

Plasmid

#170303

Purpose

A knock-out vector for the mouse Ptgfr

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Ptgfr gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh3

Plasmid

#168987

Purpose

Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3566 (pDEST15_Spt6 1223-1451)

Plasmid

#91814

Purpose

Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deleted residues 1-1222

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH1141 (pET151_Spt6 1247-1451)

Plasmid

#91815

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

deleted residues 1-1246

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT7gRNA-smyhc1_977

Plasmid

#140867

Purpose

sgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

zebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Synthetic, Zebrafish)

Use

In vitro transcription of sgrnas

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pT7gRNA-ttn.1_C3

Plasmid

#140866

Purpose

sgRNA synthesis vector for ttn.1_C3 (zebrafish titin .1).

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

zebrafish ttn.1_C3 sgRNA for in vitro transcription (ttn.1 Synthetic, Zebrafish)

Use

In vitro transcription of sgrnas

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-2

Plasmid

#83935

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-3

Plasmid

#83936

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC2-GPT1-3xFLAG

Plasmid

#163446

Purpose

lentiviral overexpression vector for human GPT1

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT1 (GPT Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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D78_Ld_362280

Plasmid

#135891

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

transmembrane emp24 domain-containing protein

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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D86_Ld_260650

Plasmid

#135899

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Protein of unknown function (DUF2012), putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLB(N)CX-FLAG-CH3

Plasmid

#133718

Purpose

Retroviral expression vector driving CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

Depositor

James A. DeCaprio

Article

Borger et al J Virol. 2006 May . 80(9):4292-303.

Insert

EP300 (EP300 Human)

Use

Retroviral

Tags

Flag

Mutation

partial (not complete) p300 fragment, CH3 domain …

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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D76_Ld_303190

Plasmid

#135889

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Translocon-associated protein beta (TRAPB), putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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