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We narrowed to 63,901 results for: cloning

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Showing: 9941 - 9960 of 63901 results

  6. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

    Plasmid
    #162949
    Purpose
    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors
    Depositor
    Dominic Esposito
    Publication
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
    Use
    Synthetic Biology
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162949-plasmid-map-sequence-id-330549

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  9. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Publication
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

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  11. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  13. pCfB2188

    Plasmid
    #67296
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.
    Depositor
    Irina Borodina
    Publication
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67296-plasmid-map-sequence-id-124518

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  14. p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K

    Plasmid
    #214460
    Purpose
    Gateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CSNK1G3 (CSNK1G3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214460-plasmid-map-sequence-id-425206

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  15. p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K

    Plasmid
    #214458
    Purpose
    Gateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CSNK1G1 (CSNK1G1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214458-plasmid-map-sequence-id-425205

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  16. p667-UBC-STK17A-V5-miniTurbo_IDG-K

    Plasmid
    #214466
    Purpose
    Gateway destination clone of STK17A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    STK17A-V5-miniTurbo (STK17A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214466-plasmid-map-sequence-id-425202

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  17. p663-UBC-miniTurbo-V5-PRPF4B_IDG-K

    Plasmid
    #214463
    Purpose
    Gateway destination clone of PRPF4B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PRPF4B (PRPF4B Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214463-plasmid-map-sequence-id-425201

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  18. p663-UBC-miniTurbo-V5-STK19_IDG-K

    Plasmid
    #214465
    Purpose
    Gateway destination clone of STK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-STK19 (STK19 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214465-plasmid-map-sequence-id-423215

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  19. pAG Retro Puro Empty

    Plasmid
    #234458
    Purpose
    Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.
    Depositor
    Melina Fan, Addgene Research Program
    Type
    Empty backbone
    Use
    Retroviral
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234458-plasmid-map-sequence-id-464639

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Showing: 9941 - 9960 of 63901 results