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Plasmid#228401PurposeEncodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1DepositorInsertSREBP1 (SREBF1 Human)
UseTagsmNeonGreen2xExpressionMammalianMutationdeleted N terminal activation domain (amino acids…PromoterEF1aAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pVHed02
Plasmid#82352PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCL1177
Plasmid#217961PurposeDual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.DepositorInsertHistone H3 (HIST2H3PS2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVHed07
Plasmid#82350PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetO(3G)-WPRE
Plasmid#186206PurposetTA dependent gene expression vectorDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterTetO(3G)Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 mRuby
Plasmid#216225PurposeTDP-43 with a C-terminal mRuby tag for expression in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseTagsmRubyExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1271
Plasmid#217963PurposeMammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.DepositorInsertImp5 (IPO5 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1316
Plasmid#217958PurposeExpression of human H4 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 mCerulean
Plasmid#216227PurposeTDP-43 with a C-terminal mCerulean tag for expression in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1161
Plasmid#217964PurposeFor RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFP, APEXExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1313
Plasmid#217955PurposeExpression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 mRuby K84ONBK
Plasmid#216226PurposeTDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.DepositorInsertTransactive response DNA binding protein of 43 kDa (TARDBP Human)
UseTagsExpressionMammalianMutationLysine 84 mutated to a TAG stop codonPromoterCMV PromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1318
Plasmid#217960PurposeExpression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
UseTagseGFPExpressionMammalianMutationL63A, F66A, I71APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1317
Plasmid#217959PurposeExpression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
UseTagseGFPExpressionMammalianMutationGGG insertion between V65 and F66PromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1315
Plasmid#217957PurposeExpression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationF84A, L92A, Y99APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1314
Plasmid#217956PurposeExpression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationGGG insertion between A95 and C96PromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
Plasmid#90009PurposeSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBPDepositorInsertsFKBP-linker-Myc-NBirA*
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_Myc-NBir-FKBP
Plasmid#90003PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer
Plasmid#90013PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-DicerDepositorInsertsCBirA*-Ago2
NBirA*-Dicer
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only