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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 491 - 500 of 28382 results

  1. pJK619

    Plasmid
    #72536
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72536-plasmid-map-sequence-id-132627

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  5. pJB158

    Plasmid
    #202601
    Purpose
    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.
    Depositor
    Athanasios Typas
    Article
    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202601-plasmid-map-sequence-id-412308

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  8. TR-5'UTR-HP-GFP

    Plasmid
    #80594
    Purpose
    Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
    Depositor
    Aravind Asokan
    Article
    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12
    Insert
    GFP
    Use
    AAV
    Expression
    Mammalian
    Mutation
    Inserted 30 nt hairpin which is targeted by Csy4 …
    Promoter
    CBA
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    80594-plasmid-map-sequence-id-152065

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  10. pJK130

    Plasmid
    #71129
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed valine-319 to phenylalanine
    Promoter
    T7
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71129-plasmid-map-sequence-id-131030

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Showing: 491 - 500 of 28382 results