We narrowed to 15,041 results for: pET
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Plasmid#222192PurposeBacterial expression of mouse PTER-H26F proteinDepositorAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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pET-dCas9-FCPF
Plasmid#220133PurposeExpresses dCas9-FCPF in E.coliDepositorInsertdCas9-FCPF (cas9 Synthetic, S. pyogenes)
UseTags6xHisExpressionBacterialMutationFCPF sequence inserted on C-terminalPromoterT7Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIV-Chondromyces
Plasmid#225078PurposeExpresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Chondromyces)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanI-101
Plasmid#225065PurposeExpresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanI-101)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mBlueberry(-9)
Plasmid#219416PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmBlueberry2(-9)
UseTagsMGHHHHHGGExpressionBacterialMutationK137E, K199E, R234EPromoterT7Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL2del
Plasmid#216742PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [LEDSGYMA…PromoterAvailable SinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-FKBP12-Sm
Plasmid#217004PurposeExpression of FKBP12-Linker-Small BiTDepositorInsertFKBP12-Linker-Small BiT
UseTagsSplit Nanoluciferase SmallBiTExpressionBacterialMutationPromoterAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-FRB-Lg
Plasmid#216981PurposeExpression of FRB-Linker-Large BiTDepositorInsertFRB-Linker-Large BiT
UseTagsSplit Nanoluciferase LargeBiTExpressionBacterialMutationPromoterAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-NIR-FbLAG30
Plasmid#220748PurposeNIR-FbLAG30 expression in bacteria.DepositorInsertNIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionBacterialMutationFor bacterial cytoplasmic expression, the pelB si…PromoterT7Available SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO-LPQTG-pET
Plasmid#221851PurposeExpresses SUMO-2 with sortase motif and His tagDepositorAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-E169A
Plasmid#222190PurposeBacterial expression of mouse PTER-E169A proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-D298A
Plasmid#222191PurposeBacterial expression of mouse PTER-D298A proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-H28F
Plasmid#222193PurposeBacterial expression of mouse PTER-H28F proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-H201F
Plasmid#222194PurposeBacterial expression of mouse PTER-H201F proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-R233A
Plasmid#222197PurposeBacterial expression of mouse PTER-R233A proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-H300A
Plasmid#222198PurposeBacterial expression of mouse PTER-H300A proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-L255A
Plasmid#222199PurposeBacterial expression of mouse PTER-L255A proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-L260G
Plasmid#222200PurposeBacterial expression of mouse PTER-L260G proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-T258G
Plasmid#222201PurposeBacterial expression of mouse PTER-T258G proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-R304K
Plasmid#222202PurposeBacterial expression of mouse PTER-R304K proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-Y65F
Plasmid#222203PurposeBacterial expression of mouse PTER-Y65F proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-Y263F
Plasmid#222204PurposeBacterial expression of mouse PTER-Y263F proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-Q249A
Plasmid#222216PurposeExpress DDX53 mutant Q249A protein in bacteriaDepositorAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-K272A
Plasmid#222215PurposeExpress DDX53 mutant K272A protein in bacteriaDepositorAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-E377Q
Plasmid#222217PurposeExpress DDX53 mutant E377Q protein in bacteriaDepositorAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-C675S
Plasmid#216756PurposeInvestigation of the potential interaction of the FeoB Cys675 residue with ferrous ironDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationAmino acid C675 is substituted with S.PromoterAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-C429S
Plasmid#216755PurposeInvestigation of the potential interaction of the FeoB Cys429 residue with ferrous ironDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationAmino acid C429 is substituted with S.PromoterAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-D123N
Plasmid#216754PurposeProduction of a FeoB that is unable to bind GTP for a negative control in GTPases assaysDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationAmino acid D123 is substituted with N.PromoterAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41SIII-ATF6(1-373)
Plasmid#171074PurposeExpresses of ATF6α(1-373) in bacteriaDepositorAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Ideonella-bGSDM-WELQ
Plasmid#222098PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.DepositorInsertIdeonella-bGSDM-WELQ ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3a-XlH3[ChEx]
Plasmid#221200PurposeXenopus laevis histone H3 with Nterminal tail swapped for ChEx domain of ScChd1.DepositorInsertXl-H3[ChEx]
UseTagsExpressionBacterialMutationPromoterAvailable SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Xl-H3∆Nterm[R42]
Plasmid#221201PurposeXenopus laevis histone H3 with N-terminal tail deleted.DepositorInsertXl-H3∆Nterm[R42]
UseTagsExpressionBacterialMutationPromoterAvailable SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-empty
Plasmid#221332PurposeProtein overexpression in bacteriaDepositorInsert6xHis-3xFKBP empty scaffold
UseTagsExpressionBacterialMutationPromoterT7Available SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-Empty-cleavableELP
Plasmid#219401PurposeInsert protease recognition site with BsaI to make custom protease-responsive elastin-like polypeptide (ELP)DepositorInsertEmpty-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-FXa-cleavableELP
Plasmid#219403PurposeFactor Xa-responsive elastin-like polypeptide (ELP)DepositorInsertFXa-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-Thr-cleavableELP
Plasmid#219405PurposeThrombin-responsive elastin-like polypeptide (ELP)DepositorInsertThr-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmN-C47A
Plasmid#221367PurposeExpress C47A ChmN in E. coliDepositorInsertchmN-C47A
UseTagsHis6ExpressionBacterialMutationchanged Cys 47 to AlaPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL4del
Plasmid#216744PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5del
Plasmid#216745PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 5 (OL5) by examining the variant lacking OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 5 (OL5) with the sequence of [ATFAA] i…PromoterAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL1del
Plasmid#216741PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [INIGGALQ…PromoterAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3L-TwStrep
Plasmid#208649Purposefor E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949DepositorInserthuman RPL3L (ENST00000268661) (RPL3L Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationcontain rs753338989, rs746044959, rs752134949, pr…PromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24
Plasmid#208621Purposefor E. coli expression of Flag tagged human RBM24 (ENST00000379052)DepositorInserthuman RBM24 (ENST00000379052) coding region (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en1dFnCas9GFP
Plasmid#201950PurposeExpression of en1dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en1dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1369R on FnCas9PromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en15dFnCas9GFP
Plasmid#201951PurposeExpression of en15dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en15dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1603H on FnCas9PromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_E192K_D193K
Plasmid#214322PurposeProtein overexpression in bacteriaDepositorAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_I48N
Plasmid#214323PurposeProtein overexpression in bacteriaDepositorAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-4_sheep_catalytic domain
Plasmid#214331PurposeProtein overexpression in bacteriaDepositorInsertCASP4
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 5W
Plasmid#213623PurposeBacterial expression of SUMO-tagged rat syt1 5WDepositorInsertsyt1 5W (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CPromoterAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 s7+
Plasmid#213624PurposeBacterial expression of SUMO-tagged rat syt1 s7+DepositorInsertsyt1 s7+ (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationL171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…PromoterAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only