-
Plasmid#20974DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
4L1M
Plasmid#60086PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHERC2:YTC007-A11:C225804 (HERC2 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
spoTD297A_NS1
Plasmid#100915Purposeexpresses spoTD297A (from native promoter) from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertsynpcc7942_1377 (spoT)
UseCyanobacteria cloning vectorTagsExpressionMutationChanged D 297 to APromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
relA+_NS1
Plasmid#100916Purposeexpresses E.coli relA+ from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertRelA fragment from E.coli
UseCyanobacteria cloning vectorTagsFLAGExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC
Plasmid#101828Purposedrives expression of kaiBC from an rpaA-independent promoterDepositorInsertkanamycin casette and p0050 promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::glgP_gap1
Plasmid#101845PurposeReplaces native promoter of glgP_gap1 with Ptrc promoterDepositorInsertCmR casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::zwf_fbp_opcA
Plasmid#101846PurposeReplaces native promoter of zwf_fbp_opcA with Ptrc promoterDepositorInsertsp/st resistance casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::gnd
Plasmid#101844Purposedrives expression of gnd using Ptrc promoterDepositorInsertKan casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
zwf-fbp-opcA-
Plasmid#101839Purposedeletes zwf_fbp_opcA locusDepositorInsertKanamycin casette (in place of synpcc7942_2333-2335 operon)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPROEX-TwStrp-FTO
Plasmid#190265PurposeExpresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.DepositorInsertFTO (FTO Human)
UseTags6xHis and Twin-StreptagExpressionBacterialMutationPromoterTrcAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
LRG2.1-CyOFP1
Plasmid#124771Purpose3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KO (KanR, 186)
Plasmid#45985DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTrig-CopA
Plasmid#104578PurposeCopy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under CopA promoter for copy increase.DepositorInsertPCopA-RiboJ-B0034-repL
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MLV Gag-YFP
Plasmid#1813DepositorInsertGag (gag MuLV)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceMay 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWY
Plasmid#112906PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KC (KanR, PhiC31)
Plasmid#45982DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAH69
Plasmid#173782PurposeThe pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.DepositorInsertHK022 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
NXPE1_pcDNA3.4-TOPO
Plasmid#226841PurposeExpresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.DepositorInsertNXPE1 (NXPE1 Human)
UseTagsx6 HIS and an IL2 secretion signal both N termina…ExpressionMammalianMutationCodon optimized for expression in human cellsPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCXI Neo DsRed-LC3-GFP
Plasmid#31183DepositorInsertDsRed-LC3-GFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2011AvailabilityAcademic Institutions and Nonprofits only