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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,139 results for: gibson assembly

Showing: 981 - 1000 of 1139 results

  1. pHRSV_CHARMS-DHF

    Plasmid
    #233922
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed Helical Filament)
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-DHF
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233922-plasmid-map-sequence-id-469215

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  2. pHRSV_CHARMS_bDD

    Plasmid
    #233931
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-dDD contains the bacterial death domain from Nostoc.
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-bDD
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233931-plasmid-map-sequence-id-469205

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  7. pFP.E222

    Plasmid
    #138248
    Purpose
    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138248-plasmid-map-sequence-id-273451

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  8. pMMK ENTR 1 CMV mAG beta-catenin

    Plasmid
    #206258
    Purpose
    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    mAG Ctnnb1 (Ctnnb1 Mouse)
    Use
    Multimate/gateway entr 1
    Tags
    monomeric Azami Green
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206258-plasmid-map-sequence-id-406391

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  9. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Available Since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

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  11. pBD21_037

    Plasmid
    #109154
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    iRFP720
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109154-plasmid-map-sequence-id-209237

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  12. pZH509

    Plasmid
    #102664
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102664-plasmid-map-sequence-id-199389

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  13. pBD21_033

    Plasmid
    #109150
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    Sirius
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109150-plasmid-map-sequence-id-209216

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  14. pBD21_036

    Plasmid
    #109153
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    mKO2
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109153-plasmid-map-sequence-id-209235

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  15. pBD21_034

    Plasmid
    #109151
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    TagBFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    109151-plasmid-map-sequence-id-220104

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  17. pZH512

    Plasmid
    #102666
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102666-plasmid-map-sequence-id-199387

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  18. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

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  19. pHRSV_CHARMS-2xTIR

    Plasmid
    #233930
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-2xTIR which contain a tandem dimer of TIR domains
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-2xTIR
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233930-plasmid-map-sequence-id-484962

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Showing: 981 - 1000 of 1139 results