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pcDNA3.1-α-COPI-WD40

Plasmid

#180758

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Leu181Lys, Leu…

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM S297A

Plasmid

#169983

Purpose

Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, S297A

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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cmv hTid Long H121Q

Plasmid

#13708

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Long H121Q (DNAJA3 Human)

Expression

Mammalian

Mutation

H121Q

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 M77T

Plasmid

#166818

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

M77T

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A54T

Plasmid

#166815

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

A54T

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2x/b delta6

Plasmid

#47587

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2x/b delta6 (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pOTTC2147 - pAAV nEF ConFon hChR2(H134R)-mCherry

Plasmid

#202545

Purpose

An INTRSECT-ready adeno-associated viral vector expressing channelrhodopsin2-mCherry fusion (Cre-On and Flp-On)

Depositor

Marisela Morales, Christopher Richie

Article

Barbano et al Nat Commun. 2025 Sep 26;16(1):8500. doi:

Insert

hChR2(H134R)-mCherry

Use

AAV

Promoter

nEF

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pOTTC2234 - pAAV Nestin GFP-iCre

Plasmid

#228443

Purpose

An adeno-associated viral vector to express GFP-iCre fusion protein under the human Nestin 2nd intron enhancer.

Depositor

Christopher Richie

Article

Ribeiro Gomes et al Cell Rep. 2025 Dec 21;45(1):116756. doi:

Insert

GFP-iCre

Use

AAV

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wtΔKDEL YD/FL

Plasmid

#214805

Purpose

Mammalian expression of human CALR wtΔKDEL YD/FL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR wtΔKDEL YD/FL (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL-V5

Plasmid

#214794

Purpose

Mammalian expression of human MPL-V5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL (MPL Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR ins5-His

Plasmid

#214796

Purpose

Baculovirus expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Tags

His

Expression

Insect

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wt

Plasmid

#214797

Purpose

Mammalian expression of human CALR wt

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wt (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR del52

Plasmid

#214798

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52 (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5

Plasmid

#214799

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR WTΔKDEL

Plasmid

#214790

Purpose

Mammalian expression of human CALR wtΔKDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wtΔKDEL (CALR Human)

Tags

non

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-50aa-puro

Plasmid

#248153

Purpose

Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 50 residues of the cytoplasmic tail.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Mutation

Mutagenesis to insert a stop codon at aa645 in FC…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-16aa-puro

Plasmid

#248152

Purpose

Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 16 residues of the cytoplasmic tail.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Mutation

Mutagenesis to insert a stop codon at aa611 in FC…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-93aa-puro

Plasmid

#248154

Purpose

Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 93 residues of the cytoplasmic tail.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Mutation

Mutagenesis to insert a stop codon at aa688 in FC…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-H4-BioID2-HA

Plasmid

#232026

Purpose

mammalian expression (CMV promoter) of histone H4 fused to BioID2-HA

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

H4 (H4C14 Human)

Tags

BioID2-HA

Expression

Mammalian

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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