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p6721 MSCV-IP N FlagHA 16E6 8S9A10T

Plasmid

#44153

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

E6 8S9A10T mutant

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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p6722 MSCV-IP N FlagHA 16E6 I128T

Plasmid

#44154

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

HPV16 E6 I128T mutant

Promoter

MSCV LTR

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
nPEX-LV

Plasmid

#12119

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Mutation

noncatalytic fragment of MMP2 amplified via PCR, …

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
Ad-shMKP-3

Plasmid

#131530

Purpose

Silence the MKP-3 expression

Depositor

Haiyan Xu

Article

Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

Insert

Short hairpin mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-MsCHI

Plasmid

#73392

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pGFP-Ub-VPS13D_dN

Plasmid

#176465

Purpose

express VPS13D lacking N-terminal 117 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCGN-ELK-1 R mut

Plasmid

#27159

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the R repression domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

R box mutant: K249A/V250A/E251A

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only
pBDT

Plasmid

#62660

Purpose

Targeting vector for PITT landing pad knock-in into the Rosa26 locus

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

eGFP
neomycin

Use

Mouse Targeting

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Hoxd13 (CT#441)

Plasmid

#13985

Depositor

Cliff Tabin

Article

Goff et al Development. 1997 Feb . 124(3):627-36.

Insert

Hoxd-13 (HOXD13 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Synthetic, Chicken)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pIS47 C20orf139 3'UTR

Plasmid

#14489

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

C20orf139 3'UTR (SRXN1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z18

Plasmid

#120061

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330)

Plasmid

#87738

Purpose

Bacterial expression of the VSL domain of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-279

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-U63-tgRNA-Rev

Plasmid

#112811

Purpose

gRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in Drosophila

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pACT2 V1H (P#663)

Plasmid

#14665

Depositor

Matija Peterlin

Article

Geyer et al Mol Biol Cell. 2002 Jun . 13(6):2045-56.

Insert

V1H (ATP6V1H Human)

Tags

Gal4 AD

Expression

Yeast

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax(TadA E59A, TadA*E59A)

Plasmid

#125649

Purpose

pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRU205

Plasmid

#167685

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU321

Plasmid

#167681

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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