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pMSCVII-tdTomato

Plasmid

#162752

Purpose

Retroviral (MSCV) expression of td Tomato fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

tdTomato

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPaxillin-PSmOrange

Plasmid

#31923

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Paxillin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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Mouse NHERF-1

Plasmid

#32950

Depositor

Edward Weinman

Article

Weinman et al Biochim Biophys Acta. 1999 Oct 6;1447(1)

Insert

Slc9a3R1 (Nherf1 Mouse)

Tags

none

Expression

Bacterial

Mutation

none

Promoter

t3

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pDA040.BLD7-mRFP

Plasmid

#206800

Purpose

EL222_D7_mRFP

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

BLD7.mRFP

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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EcRho

Plasmid

#113121

Purpose

Expresses E. coli Rho

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

Rho

Tags

MGH

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP47-2A(sfGFP)

Plasmid

#126991

Purpose

Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJBL3752

Plasmid

#128809

Purpose

Fluoride riboswitch-regulated sfGFP

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE myc-Raptor S722A/S792A

Plasmid

#18117

Depositor

Reuben Shaw

Article

Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

Insert

Raptor (RPTOR Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S722A, S792A

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
PECAM-eGFP

Plasmid

#14688

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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Human USP30 (64-502, construct 2, MG-26-18)

Plasmid

#110744

Purpose

Bacterial expression of human USP30 (construct 2)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBbB2a-GFP

Plasmid

#35343

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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FIRESFLIP-PBCS-E670

Plasmid

#228701

Purpose

Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pVimentin-PSmOrange

Plasmid

#31922

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Vimentin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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p104_gRNA_Sp_CD3e

Plasmid

#213780

Purpose

Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sp gRNA

Use

CRISPR and Lentiviral

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-NLS-M-MLV-RT

Plasmid

#181804

Purpose

pET28a-NLS-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

NLS-M-MLV-RT

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Su(fu) (CT#99)

Plasmid

#13856

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Insert

Su(fu) (SUFU Chicken)

Tags

GST

Expression

Bacterial

Mutation

Nco I fragment from full length; ~2kbp- ATG to st…

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
MYC-MST1

Plasmid

#8847

Depositor

Joseph Avruch

Article

Lin et al J Biol Chem 2002 Dec 13;277(50):47991-80

Insert

MST1 (Stk4 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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TR-GFP

Plasmid

#80591

Purpose

Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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RP4-7

Plasmid

#79811

Purpose

Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid

Depositor

Michael Hynes

Article

Quandt et al Plasmid. 2004 Jul;52(1):1-12.

Insert

Chloramphenicol acetyltransferase

Use

Helper plasmid for mobilization

Promoter

Own promoter

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-5X2-RalBP1

Plasmid

#37245

Depositor

Christopher Counter, David Kashatus

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

RalBP1 (RALBP1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only