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pCRISPaint-T2A-Gal80-3xP3-RFP

Plasmid

#127562

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal80

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal80-3xP3-RFP

Plasmid

#127562

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal80

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG2

Plasmid

#112814

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdGFP

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG2

Plasmid

#112814

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdGFP

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3_2FLAG-AKAP79ΔCN

Plasmid

#183451

Purpose

Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

aa337-343 deleted

Promoter

CMV

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_2FLAG-AKAP79ΔCN

Plasmid

#183451

Purpose

Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

aa337-343 deleted

Promoter

CMV

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-V96 ELP

Plasmid

#67853

Purpose

GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

Depositor

Andrew MacKay

Article

Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

Insert

GFP fused to ELP (VPGVG) repeated 96 times

Tags

GFP fused to N-terminal of ELP

Expression

Mammalian

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-V96 ELP

Plasmid

#67853

Purpose

GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

Depositor

Andrew MacKay

Article

Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

Insert

GFP fused to ELP (VPGVG) repeated 96 times

Tags

GFP fused to N-terminal of ELP

Expression

Mammalian

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM-sgMUC1_dual-MTS-3x-Set I

Plasmid

#162763

Purpose

Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3

Expression

Mammalian

Promoter

U6

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-sgMUC1_dual-MTS-3x-Set I

Plasmid

#162763

Purpose

Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3

Expression

Mammalian

Promoter

U6

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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TrpLE-M-nMSP1E3D1

Plasmid

#179949

Purpose

Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

Depositor

Kerney Glover

Article

Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

Insert

TrpLE-M-nMSP1E3D1

Tags

TrpLE

Expression

Bacterial

Mutation

Native Trp residues have been mutated to Phe resi…

Promoter

T7

Available Since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TrpLE-M-nMSP1E3D1

Plasmid

#179949

Purpose

Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

Depositor

Kerney Glover

Article

Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

Insert

TrpLE-M-nMSP1E3D1

Tags

TrpLE

Expression

Bacterial

Mutation

Native Trp residues have been mutated to Phe resi…

Promoter

T7

Available Since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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