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pKB33 (pUC19-KANMX-tetO7-CYCpr)

Plasmid

#210472

Purpose

Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Insert

KanMX-TetO7-CYCpr cassette

Use

Synthetic Biology

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCA17-CRISPR-Tag (11XTS1) for dSpCas9

Plasmid

#199448

Purpose

CRISPR-Tag sequence that can be efficiently bound by dSpCas9

Depositor

Baohui Chen

Publication

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

CRISPR-Tag

Use

CRISPR

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-mNeonGreen(2x)

Plasmid

#197919

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Publication

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

glycosylphosphatidylinositol-mNeonGreen-NeonGreen

Expression

Mammalian

Mutation

none

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-mGreenLantern(2x)

Plasmid

#197923

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Publication

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

glycosylphosphatidylinositol-mGreenLantern-mGreenLantern

Expression

Mammalian

Mutation

none

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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ATOH1_pUC18

Plasmid

#191250

Purpose

Encodes the sequence for the ATOH1 gene (human)

Depositor

Chen Davidovich

Publication

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

ATOH1 (ATOH1 Human)

Expression

Bacterial

Promoter

lac promoter

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO-perox-mCer

Plasmid

#185603

Purpose

Lentiviral construct for expression of mCerulean targeted to peroxisomes

Depositor

Jarrod Johnson

Publication

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mCer

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_KanR neo

Plasmid

#167861

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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L1_UBE2:mS-Lt-Ck4

Plasmid

#136132

Purpose

L1 in position 4, mark cell outline with mScarletI

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mScarletI-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b-sad1-C63A

Plasmid

#90302

Purpose

protein expression

Depositor

Christine Guthrie

Publication

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

C63A

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-RIIα

Plasmid

#168492

Purpose

Mammalian expression of mouse PKA regulatory subunit Iiα, untagged.

Depositor

Haining Zhong

Publication

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse PKA regulatory subunit IIα, untagged

Expression

Mammalian

Available since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTrc99S-ssDsbA-PD-4xDQNAT

Plasmid

#128399

Purpose

Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

H. influenzae protein D

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pDHAS

Plasmid

#160920

Purpose

Dihydroxyacetone synthase (DHAS) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Dihydroxyacetone synthase promoter

Use

Synthetic Biology

Mutation

BsaI and BbsI domesticated

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-PK1Dexp

Plasmid

#130834

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Publication

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available since

June 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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SNX27-PX (156-265)

Plasmid

#119106

Purpose

Bacterial expression of human phox homology (PX) domain, SNX27-PX (156-265)

Depositor

Brett Collins

Publication

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX27-PX (156-265) (SNX27 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-nirButterfly

Plasmid

#136590

Purpose

Expression of a genetically-encoded voltage indicator nirButterfly

Depositor

Vladislav Verkhusha

Publication

Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nirButterfly

Expression

Mammalian

Promoter

CAG

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PX458_CIC_iso1_2

Plasmid

#135744

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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JB24

Plasmid

#128906

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Publication

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab8AQL-BirA*-HA-MAO (RAB8A Human)

Expression

Mammalian

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB10

Plasmid

#128917

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Publication

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab18SN-BirA*-HA-MAO (RAB18 Human)

Expression

Mammalian

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB2

Plasmid

#128899

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Publication

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab2ASN-BirA*-HA-MAO (RAB2A Human)

Expression

Mammalian

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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LEPG_shSik3.308

Plasmid

#125878

Purpose

Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Publication

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

shRNA targeting Sik3

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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