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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,351 results for: multi

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Showing: 10061 - 10080 of 18351 results

  2. pHL 1278

    Plasmid
    #53018
    Purpose
    AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1
    Depositor
    Han Lim
    Publication
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Inserts
    mCherry
    GFP
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pAG426-GAL-UBQLN2

    Plasmid
    #181708
    Purpose
    Galactose inducible expression of UBQLN2
    Depositor
    Alejandro Chavez
    Insert
    UBQLN2 (UBQLN2 Human)
    Expression
    Yeast
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    181708-plasmid-map-sequence-id-436183

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  13. GPR156-DuET

    Plasmid
    #213267
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR156 (GPR156 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213267-plasmid-map-sequence-id-431807

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  14. AGTR2-DuET

    Plasmid
    #213184
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AGTR2 (AGTR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    213184-plasmid-map-sequence-id-431748

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  15. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Publication
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  19. pMT450

    Plasmid
    #139390
    Purpose
    Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Publication
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
    Use
    Synthetic Biology
    Promoter
    Bile Acid inducible Promoter
    Available since
    Jan. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    139390-plasmid-map-sequence-id-313840

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  20. pS44i8GM

    Plasmid
    #207527
    Purpose
    Conditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Publication
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
    Use
    CRISPR; Pseudomonas vector
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207527-plasmid-map-sequence-id-409603

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Showing: 10061 - 10080 of 18351 results