We narrowed to 36,793 results for: KAN

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pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

Plasmid

#62131

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR and Gateway Cloning

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK084

Plasmid

#65191

Purpose

Encodes KanR-ColE1 as a Type 8 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

KanR-ColE1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFGL822

Plasmid

#58225

Purpose

For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

phosphinothricin acetyl transferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pPd4E-ISH

Plasmid

#61700

Purpose

Plum eIF4E ihp-RNA construct

Depositor

Lining Tian

Article

Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

Insert

PdeIF4E

Use

RNAi

Promoter

35S

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF8-N-terminus(1-111)

Plasmid

#64671

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only N-terminus amino acids 1 to 111

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pDSAR-Cre-F2A-I-SceI

Plasmid

#62302

Purpose

Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase, I-SceI nuclease

Expression

Insect

Promoter

vasa

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pC-SUR-YR

Plasmid

#61768

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Sur gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

Magnaporthe oryzae ILV promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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DHFR

Plasmid

#64200

Purpose

Expression of E. coli DHFR

Depositor

Vern Schramm

Article

Wang et al J Am Chem Soc. 2014 Jun 11;136(23):8333-

Insert

Dihydrofolate reductase

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
IFP2.0-H2B-6

Plasmid

#56273

Purpose

Localization: Nucleus/Histones, Excitation: 684, Emission: 708

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

IFP2.0

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP-LaminA-C-18

Plasmid

#56226

Purpose

Localization: Nuclear Envelope, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LaminA (LMNA Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCerulean3-mVenus-CASPER

Plasmid

#58162

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Type

Empty backbone

Tags

mCerulean3 and mVenus-CASPER

Expression

Mammalian

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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FusionRed-Fibrillarin-7

Plasmid

#56118

Purpose

Localization: Nucleoli, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Fibrillarin (FBL Human)

Tags

FusionRed

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pQE-WT-N4-R5-RNRV

Plasmid

#63630

Purpose

N4-R5-RNRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

N4-R5-RNRV RNAP

Mutation

L747I, N748D, L749C, M750V, F751I, Q754T, F755R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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dKeima-Red-H2B

Plasmid

#56024

Purpose

Localization: Nucleus/Histones, Excitation: 440, Emission: 616

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

mKeima-Red

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only
PS-CFP2-Farnesyl-5

Plasmid

#57227

Purpose

Localization: Membrane, Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

Farnesyl (HRAS Human)

Tags

PS-CFP2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PS-CFP2-Clathrin-15

Plasmid

#57220

Purpose

Localization: Clathrin Vesicles, Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

Clathrin (CLTB Human)

Tags

PS-CFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mRuby2-CENPB-N-22

Plasmid

#55893

Purpose

Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

CENPB (CENPB Human)

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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FusionRed-VASP-5

Plasmid

#56136

Purpose

Localization: Focal Adhesions, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

VASP

Tags

FusionRed

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PS-CFP2-Actin-7

Plasmid

#57213

Purpose

Localization: Actin, Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

PS-CFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
GFP-DroshaS300E/S302D

Plasmid

#62531

Purpose

Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to glutamic acid and serine 3…

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only