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C453-E09: attB4-Grp78p>-attB5

Plasmid

#162974

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human Grp78 promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Glucose response protein 78 promoter

Use

Synthetic Biology

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E11: attB5r-mTFP1-nostop-attB1r

Plasmid

#162942

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E11: attB4-Grp78p>-attB1r

Plasmid

#162970

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Glucose response protein 78 promoter

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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C413-E10: attB4-FerHp>-attB1r

Plasmid

#162969

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ferritin heavy chain promoter

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E29: attB5r-mKate2-nostop-attB1r

Plasmid

#162946

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Kate2 fluorescent protein

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E15: attB5r-mVenus-nostop-attB1r

Plasmid

#162943

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Adeno-miR-23b

Plasmid

#112538

Purpose

Adenovirus overexpression

Depositor

Shusheng Wang

Publication

Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

Insert

miR-23b (MIR23B Human)

Use

Adenoviral

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-WT-no charge

Plasmid

#160514

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

E337A, N338A, K341A, N342A

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 AlcA (GB1549)

Plasmid

#160584

Purpose

Ethanol inducible operator

Depositor

Diego Orzaez

Insert

AlcA

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCIS-rPAM3

Plasmid

#145803

Purpose

expresses rat PAM3 in mammalian cells

Depositor

Betty Eipper, Richard Mains

Publication

Milgram et al J Cell Biol. 1994 Jan;124(1-2):33-41. do

Insert

rat PAM3

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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myc-Miro2 (lacking TM)

Plasmid

#127614

Purpose

Human Miro2 with the transmembrane deleted

Depositor

Josef Kittler

Publication

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Miro2 (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

Transmembrane deletion

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFR093

Plasmid

#134149

Purpose

Expression of CENP-T

Depositor

Iain Cheeseman

Publication

Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

Insert

CENP-T (CENPT Human)

Tags

His

Expression

Bacterial

Mutation

aa 1-375 with T85D mutation

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI126

Plasmid

#126066

Purpose

Module 1 vector of PlaMiNGo toolkit for expression of plastid targeted GFP1-10 in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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1476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spA

Plasmid

#109315

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp

Depositor

William Lagor

Publication

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Insert

Mttp gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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LIII 1-6 Amp RC

Plasmid

#104530

Purpose

LIII backbone vector

Depositor

Thomas Lahaye

Publication

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Type

Empty backbone

Use

Ralstonia solanacearum transformation vector

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9-GFP T119D

Plasmid

#114668

Purpose

Expresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Publication

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Promoter

ATG9

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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AP1298‐1

Plasmid

#87248

Purpose

gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)

Depositor

Geraldine Seydoux

Publication

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Insert

gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNH (GB0064)

Plasmid

#68165

Purpose

Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlNH promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPMtb (GB0080)

Plasmid

#68167

Purpose

Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlMtb promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPTctp (GB0083)

Plasmid

#68169

Purpose

Provides the S. Lycopersicum translationally-controlled tumor protein homolog promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlTCTP promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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