We narrowed to 7,696 results for: Pol
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Plasmid#227780PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neurons fused to mRuby3DepositorInsertBiPOLES-mRuby3
ExpressionMammalianAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGF-bGH polyA-G
Plasmid#218902Purposegolden gate cloningDepositorInsertbGH polyA
UseSynthetic BiologyAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBAC3-POLY-pnRFP
Plasmid#201680PurposeBoost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)DepositorInsert813
ExpressionMammalianAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK005 ubi:mCerulean_ubb-polyA
Plasmid#195979PurposeExpression constructDepositorInsertubi:mCerulean
UseOtherAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-somBiPOLES-mCerulean
Plasmid#192578PurposeAAV-vector for bidirectional optogenetic manipulation of tetTag-positive neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianPromoterTRE3GVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-eGFP
Plasmid#192583PurposeAAV-vector for bidirectional optogenetic manipulation of excitatory neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianPromoterCaMKIIa(0.4)Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-Pole4 KO sgRNA
Plasmid#186935PurposePole4 KO in mouse ES cellsDepositorPublicationAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-Pole3 KO sgRNA
Plasmid#186934PurposePole3 KO in mouse ES cellsDepositorPublicationAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgPole#2/Cre
Plasmid#173610PurposeExpresses a Pole-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Pole (Pole Mouse)
UseCRISPR, Cre/Lox, and LentiviralAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgPole#1/Cre
Plasmid#173609PurposeExpresses a Pole-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Pole (Pole Mouse)
UseCRISPR, Cre/Lox, and LentiviralAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-POLBHR-eGFP
Plasmid#176064PurposeHomology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1DepositorInsertEGFP-PolB (POLB Human)
TagsEGFPExpressionMammalianMutationpUC with endogenous SapI mutated out, PAM mutatio…PromoterN/AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGST-pol1-N2-2A2
Plasmid#160991PurposeExpress a fragment of yeast pol1 proteinDepositorInsertpol1
TagsGSTExpressionBacterialMutationF58A/D62AAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-pol1-N1-2A2
Plasmid#160990PurposeExpress a fragment of yeast pol1 proteinDepositorInsertpol1
TagsGSTExpressionBacterialMutationF58A/D62AAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-POLR2E-213
Plasmid#117356PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR POLR2E-213
UseLuciferaseTagsluciferaseExpressionMammalianPromoterMurine PGK-1Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
YCe1889 HC_Kan_SV40-polyA_p11
Plasmid#100678Purposepart designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequenceDepositorInsertSV40-polyA
UseSynthetic BiologyAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
Plasmid#164615PurposeExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRenilla luciferase
Tags24xMS2v5 (3'UTR) and SunTagExpressionBacterial and MammalianPromoterTetCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA
Plasmid#232935PurposeDonor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT2A-eGFP-SV40pA with Grb10 homology arms
UseMouse TargetingAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH23 Dual G-less containing ySSA1 polyA
Plasmid#231647Purposeyeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassetteDepositorInsertSSA1 polyA termination region
UseSynthetic BiologyAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH20 Dual G-less containing yCYC1 polyA
Plasmid#231646Purposeyeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassetteDepositorInsertCYC1 polyA termination region
UseSynthetic BiologyAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA
Plasmid#218869PurposeMammalian expression of improved GABA sensor (negative change in fluorescence)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiGABASnFR2n
ExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only