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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,102 results for: cas12a

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Showing: 1001 - 1020 of 1102 results

  2. pYPQAT48

    Plasmid
    #223420
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for dicot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-Mb2Cas12a-RVRR-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223420-plasmid-map-sequence-id-452225

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  5. pYPQAT43

    Plasmid
    #223415
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR; T-dna vector
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223415-plasmid-map-sequence-id-453575

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  6. pFH103

    Plasmid
    #128208
    Purpose
    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
    Depositor
    Vladimir Nekrasov
    Publication
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128208-plasmid-map-sequence-id-252907

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  8. pYPQ230-RVR

    Plasmid
    #108861
    Purpose
    LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations
    Depositor
    Yiping Qi
    Publication
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R, K554V and Y558R, Cpf1 is rice codon optimi…
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108861-plasmid-map-sequence-id-209929

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  9. pYPQ239-RR

    Plasmid
    #108862
    Purpose
    FnCpf1 Gateway entry plasmid with N623R and K687R mutations
    Depositor
    Yiping Qi
    Publication
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N623R and K687R, Cpf1 is rice codon optimized
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108862-plasmid-map-sequence-id-274177

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  10. pY112 (pcDNA3.1-huSaCpf1)

    Plasmid
    #92269
    Purpose
    Expression of humanized SaCpf1
    Depositor
    Feng Zhang
    Insert
    huSaCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92269-plasmid-map-sequence-id-181496

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  11. pY114 (pcDNA3.1-huPgCpf1)

    Plasmid
    #92273
    Purpose
    Expression of humanized PgCpf1
    Depositor
    Feng Zhang
    Insert
    huPgCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92273-plasmid-map-sequence-id-181493

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  12. pY122 (pcDNA3.1-huCPbCpf1)

    Plasmid
    #92298
    Purpose
    Expression of humanized CPbCpf1
    Depositor
    Feng Zhang
    Insert
    huCPbCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92298-plasmid-map-sequence-id-181648

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  13. pY118 (pcDNA3.1-huLb4Cpf1)

    Plasmid
    #92294
    Purpose
    Expression of humanized Lb4Cpf1
    Depositor
    Feng Zhang
    Insert
    huLb4Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92294-plasmid-map-sequence-id-181497

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  17. pCSN071

    Plasmid
    #166729
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Publication
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166729-plasmid-map-sequence-id-332207

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  18. pJK520

    Plasmid
    #155386
    Purpose
    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Availability
    Academic Institutions and Nonprofits only
    155386-plasmid-map-sequence-id-309003

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  19. pCSN072

    Plasmid
    #166730
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Publication
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166730-plasmid-map-sequence-id-332216

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  20. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    155388-plasmid-map-sequence-id-324484

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Showing: 1001 - 1020 of 1102 results