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pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

Plasmid

#131684

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1

Depositor

Christopher Richie

Publication

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Promoter

EF1a and mU6-LSL (Cre dependent)

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-spo5DSR

Plasmid

#48983

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Publication

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA

Plasmid

#37088

Purpose

Expresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibition

Depositor

Bernardo Sabatini

Publication

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

NpHR3.0-EYFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cHAIAA7-spo5DSR

Plasmid

#49873

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Publication

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pJPC263

Plasmid

#234530

Purpose

Cre/Lox P1 Recombinase switch; plant T-DNA

Depositor

Daniel Voytas

Publication

Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

Insert

RUBY

Expression

Plant

Mutation

none

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP

Plasmid

#48988

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Publication

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA

Plasmid

#48989

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Publication

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA tag

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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Smarce1-Ccnb1-mitotic degron [R42A mutant] donor template (Flox-PGK-NeoR)

Plasmid

#259650

Purpose

Homologous recombination donor template to generate Smarce1-Ccnb1 mitotic degron [R42A mutant] knockin mouse embryonic stem cells

Depositor

Charles Roberts

Publication

Zhu et al Nature. 2023 Jun;618(7963):180-187. doi:

Insert

Smarce1 (Smarce1 Mouse)

Use

Mouse Targeting

Mutation

R42A mutation in Ccnb1

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pQcre1

Plasmid

#135659

Purpose

Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites.

Depositor

Andrew Tolonen

Publication

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

ppag promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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LentiX-(loxPcon-TET-DIAL-YB-TATA)-mCherry-HRasG12V-bGH-EFS-rtTA-TagBFP-WPRE

Plasmid

#246367

Purpose

loxPcon TET-DIAL Reporter Lentivirus with YB_TATA expressing mCherry-HRasG12V in the presence of DOX and rtTA and editable by Cre recombinase. Contains rtTA-TagBFP expressed divergenty.

Depositor

Kate Galloway

Publication

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mCherry-GS-HRasG12V (HRAS Human)

Use

Lentiviral and Synthetic Biology

Mutation

G12V

Available since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAW8_6XFLAG

Plasmid

#110235

Purpose

Cre/Lox C-terminal tagging construct encoding 6 copies of the FLAG epitope

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHJ42

Plasmid

#48359

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable

Depositor

George Cross

Publication

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pHJ32

Plasmid

#48361

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin selectable

Depositor

George Cross

Publication

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-8XDSR

Plasmid

#48981

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Publication

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-6XDSR

Plasmid

#48980

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Publication

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-3XDSR

Plasmid

#48976

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Publication

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-4XDSR

Plasmid

#48978

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Publication

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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AAVS1-Bxb1-LP-TC

Plasmid

#183754

Purpose

AAVS1 targeting construct with the Bxb1 landing pad cassette.

Depositor

Richard Davis

Publication

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox257

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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CLYBL-Bxb1-LP-v2-TC

Plasmid

#194327

Purpose

CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

Depositor

Richard Davis

Publication

Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

Insert

loxP-EBFP2-attP-BleoR-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMCS.71/2272.Hygro

Plasmid

#22731

Depositor

Mark Magnuson

Publication

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox71-FRT-PGK-hygro-FRT-Lox2272

Use

Cre/Lox

Mutation

This is a basal exchange vector for RMCE. The vec…

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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