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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,719 results for: plasmid for e coli

Showing: 1001 - 1020 of 1719 results

  1. pE-SUMO CRYBB2

    Plasmid
    #80749
    Purpose
    Expression of human beta B2-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBB2 (CRYBB2 Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80749-plasmid-map-sequence-id-153512

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  2. pE-SUMO CRYBB3

    Plasmid
    #80750
    Purpose
    Expression of human beta B3-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBB3 (CRYBB3 Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80750-plasmid-map-sequence-id-152180

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  3. Sc Pol3-FLAG(Ec)-pRSF405/GAL

    Plasmid
    #239198
    Purpose
    Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)
    Depositor
    Michael O'Donnell
    Insert
    Pol3 (POL3 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    E. coli codon bias (synthetic gene), but same ami…
    Promoter
    Available Since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239198-plasmid-map-sequence-id-474564

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  4. pGEX-4T_CIP2A_1-560_R522D

    Plasmid
    #217923
    Purpose
    E. coli expression of GST-tagged CIP2A_1-560 with R522D mutation
    Depositor
    Jukka Westermarck
    Insert
    CIP2A (CIP2A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    R522D (522 AGA->GAT)
    Promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    217923-plasmid-map-sequence-id-433889

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  5. pGEX-4T_CIP2A_1-560_L533E

    Plasmid
    #217924
    Purpose
    E. coli expression of GST-tagged CIP2A_1-560 with L533E mutation
    Depositor
    Jukka Westermarck
    Insert
    CIP2A (CIP2A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    L533E (533 TTG->GAG)
    Promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    217924-plasmid-map-sequence-id-433888

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  16. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

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  17. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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  18. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid
    #134924
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134924-plasmid-map-sequence-id-267600

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  19. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid
    #134923
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134923-plasmid-map-sequence-id-267593

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Showing: 1001 - 1020 of 1719 results