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pPUbc (GB0145)

Plasmid

#68170

Purpose

Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlUbc promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p2x35S (GB0222)

Plasmid

#68173

Purpose

Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

2x35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
BCD12_BC

Plasmid

#66023

Purpose

MoClo Basic Part: RBS - BiCistronic Design, medium strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD12:C]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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BCD2_BC

Plasmid

#66024

Purpose

MoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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BCD8_BC

Plasmid

#66025

Purpose

MoClo Basic Part: RBS - BiCistronic Design, low strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD8:C]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pT2A11 (GB0143)

Plasmid

#68189

Purpose

Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTE8 (GB0144)

Plasmid

#68190

Purpose

Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pUNC2001

Plasmid

#67280

Purpose

Expresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw

Depositor

Aziz Sancar

Publication

Worthington et al J Biol Chem. 2003 Oct 3;278(40):39143-54

Insert

Vibrio cholera photolyase

Tags

maltose binding protein

Expression

Bacterial

Mutation

none known

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M1A500(KaganF60)

Plasmid

#52003

Purpose

Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain

Depositor

Jonathan Kagan

Publication

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A-500

Expression

Mammalian

Mutation

changed Met 1 to Ala and deleted the C-terminal t…

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
porcupine

Plasmid

#37377

Depositor

Norbert Perrimon

Publication

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (por Fly)

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
LRRK2_1867_2161_F1908A_L1936R_V2048S_F2079G

Plasmid

#40413

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867-2161; F1908A, L1936R, V2048S, F2079G

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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LRRK2_1867_2161_F1908A_L1936R_V2048S_I2131Q

Plasmid

#40418

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867-2161; F1908A, L1936R, V2048S, I2131Q

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
LRRK2_1867_2161_F1908A_L1936R_V2048S_F2079G_I2131Q

Plasmid

#40423

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; F1908A, L1936R, V2048S, F2079G, I213…

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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LRRK2_1867_2161_L1914N_L1936Y_V2048T_F2083Y_I2131S

Plasmid

#40426

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

1867_2161; L1914N, L1936Y, V2048T, F2083Y, I2131S

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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LRRK2_1867_2161_L1916N_L1936R_L1976A_I2049A_L2092N

Plasmid

#40438

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; L1916N, L1936R, L1976A, I2049A, L209…

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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LRRK2_1867_2161_L1916N_L1976A_I2049A_V2074A_L2092N

Plasmid

#40439

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; L1916N, L1976A, I2049A, V2074A, L209…

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
LRRK2_1867_2161_L1914A_L1976Y_C2024S_C2025S_I2049Q

Plasmid

#40452

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; L1914A, L1976Y, C2024S, C2025S, I204…

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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Kek2 (Lip2)

Plasmid

#38752

Depositor

Norbert Perrimon

Publication

Musacchio et al Dev Biol. 1996 Aug 25;178(1):63-76.

Insert

Kek2 (kek2 Fly)

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
-833pSia-Luc

Plasmid

#32989

Depositor

Sergei Sokol

Publication

Fan et al Proc Natl Acad Sci U S A. 1998 May 12;95

Insert

Siamois promoter (sia1.L Frog)

Use

Luciferase

Tags

Luciferase

Promoter

Siamois

Available since

Jan. 5, 2012

Availability

Academic Institutions and Nonprofits only
pSP64T XAR1 (DM#105)

Plasmid

#15044

Depositor

Douglas Melton

Publication

Hemmati-Brivanlou et al Nature. 1992 Oct 15. 359(6396):609-14.

Insert

Xenopus activin receptor 1 (xstk2 Frog)

Expression

Bacterial

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only