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pWPXL-p150

Plasmid

#160992

Purpose

Express fulllength of mouse p150 protein

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RNF110

Plasmid

#126547

Purpose

Expresses GFP-tagged RNF110 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF110 (PCGF2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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GST-PS-Sos1 (PRM) - Cys (XSB364)

Plasmid

#78506

Purpose

Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Sos1 -Cys (PRM) (SOS1 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-BLST-Cas9-VPR

Plasmid

#115147

Purpose

Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-Cas9-VPR

Use

CRISPR

Tags

SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTHMT_hnRNPF_deltaPLD

Plasmid

#139115

Purpose

express FL hnRNPF without the prion like domain with N-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Expression

Bacterial

Mutation

delta365-415

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Bin1 N-BAR domain

Plasmid

#168280

Purpose

Express N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vector

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-267

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_R4187C

Plasmid

#187142

Purpose

mammalian cell expression of 3xFLAG-HUWE1 R4187C

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

R4187C

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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B2387_mEGFP-FKBP2x

Plasmid

#216216

Purpose

Expresses mEGFP(A206K) fused to FKBP-FKBP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

mEGFP-FKBP-FKBP (FKBP1A Human)

Tags

EGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Mili-EGFP

Plasmid

#212576

Purpose

Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Mili (Piwil2 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGLS3-5xHRE-PHLDA3(wt)

Plasmid

#72557

Purpose

Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

PHLDA3 (PHLDA3 Human)

Tags

5XHRE

Expression

Mammalian

Promoter

E1b minimal

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-gp120(BaL)-CD4

Plasmid

#98957

Purpose

Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

HIV-1 (BaL) gp120

Tags

CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pST_CAHS3_HYPDU (pBS0333)

Plasmid

#185179

Purpose

For the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS3_HYPDU

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E114I (pBS0839)

Plasmid

#185274

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E114I

Expression

Mammalian

Mutation

E114I

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR4_CELF1

Plasmid

#106098

Purpose

Entry vector for CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Use

Gateway entry vector

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-SIK3 T196E

Plasmid

#52975

Purpose

insect expression of SIK3 T196E constitutively active mutant

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

T196E constitutively active mutant

Promoter

Hsp70

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
Bnip3L RNAi pSuper-retro

Plasmid

#17468

Depositor

Wafik El-Deiry

Article

Fei et al Cancer Cell. 2004 Dec . 6(6):597-609.

Insert

Bnip3L RNAi (BNIP3L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
Twitch-3 pRSETB

Plasmid

#48204

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3A

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only
pGEMHE-TNKS-2

Plasmid

#154149

Purpose

For making TNKS-2 RNA

Depositor

Carmen Williams

Article

Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

Insert

Tankyrase 2 (TNKS2 Human)

Use

In vitro transcription

Tags

FLAG

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLV V5-Tmem214

Plasmid

#175153

Purpose

Lentiviral expression of V5-tagged mouse Tmem214

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem214 (Tmem214 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

Plasmid

#203439

Purpose

Mammalian expression of RUNX4

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

RUNX3 (RUNX3 Human)

Tags

3 x FLAG

Expression

Mammalian

Mutation

R122C

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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