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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 10261 - 10280 of 13842 results

  5. pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

    Plasmid
    #244844
    Purpose
    Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification
    Depositor
    Jennifer Doudna
    Article
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Insert
    iCas12a
    Use
    CRISPR
    Tags
    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS
    Expression
    Bacterial
    Mutation
    F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…
    Promoter
    T7
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244844-plasmid-map-sequence-id-486520

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  13. POC1550

    Plasmid
    #226497
    Purpose
    pMOBC360_VM_C1 assembled plasmid (Chloramphenicol R) containing four inserts (POC1546-POC1549) assembled in POC1525 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226497-plasmid-map-sequence-id-465770

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  14. POC1546

    Plasmid
    #226487
    Purpose
    pMOBK360_VL_N1 assembled plasmid (Kanamycin R) containing four inserts (POC1535-POC1538) assembled in POC1518 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    ssembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226487-plasmid-map-sequence-id-469009

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  15. POC1547

    Plasmid
    #226489
    Purpose
    pMOBK360_VM_N2 assembled plasmid (Kanamycin R) containing three inserts (POC1539-POC1541) assembled in POC1519 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226489-plasmid-map-sequence-id-468559

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  16. POC1548

    Plasmid
    #226490
    Purpose
    pMOBK360_VM_N3 assembled plasmid (Kanamycin R) containing two inserts (POC1542-POC1543) assembled in POC1519 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226490-plasmid-map-sequence-id-468573

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  17. POC1549

    Plasmid
    #226491
    Purpose
    pMOBK360_VR_N4 assembled plasmid (Kanamycin R) containing two inserts (POC1544-POC1545) assembled in POC1520 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226491-plasmid-map-sequence-id-465763

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  18. POC1431

    Plasmid
    #221578
    Purpose
    Assembled plasmid containing four inserts (from POC1426, POC1427, POC1428 and POC1429) assembled using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing four always-methylable internal BsaI sites
    Use
    Synthetic Biology
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    221578-plasmid-map-sequence-id-465738

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  19. pYPQAT16

    Plasmid
    #223388
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    223388-plasmid-map-sequence-id-474023

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Showing: 10261 - 10280 of 13842 results