We narrowed to 44,249 results for: his

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pET28-His6-TEV-Grb2-SH2-mCherry-AviTag

Plasmid

#214231

Purpose

Bacterial expression of SH2 domain of the Grb2 with N-terminal TEV-cleavable 6xHis tag, C-terminal mCherry, and C-terminal AviTag for Streptavidin bead functionalization for binder selections.

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

Grb2 SH2 domain (GRB2 Human)

Tags

6xHis, AviTag, and mCherry

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Plasmid

#200792

Purpose

Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Δ954-1027

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Plasmid

#200798

Purpose

Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

411-680

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

Plasmid

#169135

Purpose

E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

Coh7

Tags

3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EV-D68 2A protease; 6xHis-SUMO, low expression levels

Plasmid

#204802

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac HTB His-TEV-rDPP9(S729A)-FLAG

Plasmid

#217329

Purpose

Purification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cells

Depositor

Yifan Wang

Insert

DPP9

Tags

FLAG and His-TEV

Expression

Insect

Mutation

S729A

Promoter

polyhedrin

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG

Plasmid

#217325

Purpose

Purification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cells

Depositor

Yifan Wang

Insert

NLRP1

Tags

FLAG and His-TEV

Expression

Insect

Mutation

N61K

Promoter

polyhedrin

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

Plasmid

#217324

Purpose

Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

Depositor

Yifan Wang

Insert

NLRP1

Tags

FLAG and His-TEV

Expression

Insect

Mutation

S969A

Promoter

polyhedrin

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

Plasmid

#217320

Purpose

Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

Depositor

Yifan Wang

Insert

NLRP1

Tags

FLAG and His-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt

Plasmid

#221890

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

HNRNPA3 (HNRNPA3 Human)

Tags

6xHis-MBP-PreScission-mGFP-TEV

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01

Plasmid

#211890

Purpose

Bacterial expression of CENP-T[1-242/3D]-I3-01

Depositor

Iain Cheeseman

Insert

CENP-T[1-242/3D]-I3-01 (CENPT Synthetic, Human)

Expression

Bacterial

Mutation

T11D, T27D, T85D

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]

Plasmid

#211891

Purpose

Bacterial expression of CENP-T[1-242/3D]

Depositor

Iain Cheeseman

Insert

CENP-T[1-242/3D] (CENPT Human)

Expression

Bacterial

Mutation

T11D, T27D, T85D

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

Plasmid

#212795

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R/K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R/K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_1-640-del331-370-V5His6_AH

Plasmid

#148906

Purpose

Insect Expression of DmGIGYF_1-640-del331-370

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_1-640-del331-370 (Gyf Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmRoquin_702-819-del790-812-GB1His6_AE

Plasmid

#148623

Purpose

Bacterial Expression of DmRoquin_702-819-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del790-812 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmRoquin_702-819-del725-755-GB1His6_AE

Plasmid

#148622

Purpose

Bacterial Expression of DmRoquin_702-819-del725-755

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del725-755 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-del28-43-V5His6_Z

Plasmid

#148157

Purpose

Insect Expression of Dm4E-T_1-511-del28-43

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-del28-43 (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-FWKtoAAA-V5His6_Z

Plasmid

#148158

Purpose

Insect Expression of Dm4E-T_1-511-FWKtoAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-FWKtoAAA (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-Y10AL15A-V5His6_Z

Plasmid

#148161

Purpose

Insect Expression of Dm4E-T_1-511-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-Y10AL15A (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z

Plasmid

#148162

Purpose

Insect Expression of Dm4E-T_1-511-YLLLtoAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-YLLLtoAAAA (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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