We narrowed to 23,032 results for: his
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Plasmid#113387PurposeExpress human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal claspDepositorInserthuman beta 1 ectodomain
TagsHis tagExpressionMammalianPromoterCMVAvailable SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAD/CMV/V5-DEST-Rac1-2G
Plasmid#66112PurposeSecond generation Rac1 FRET biosensor for adenovirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseAdenoviralTagsHis-MycExpressionMammalianPromoterCMVAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
Tags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TmCA-P(11)4
Plasmid#185783PurposeEncodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertTmCA-P(11)4
TagsHis-tagExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
Tags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
spa-GCaMP6s
Plasmid#67556Purposephotoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6s
TagsHIS-tagExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…Available SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PSGU
Plasmid#59738PurposeExpresses a.a. 417-887 of human ZO-1DepositorInsertzonula occludens-1 (TJP1 Human)
TagsHIS tag and thrombin clvgExpressionBacterialPromoterT7Available SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
PM18033-PLS-KP6_rpoA-U
Plasmid#190987PurposeTest the U-to-C editing activity of KP6 on AtrpoA editing site, in human cellsDepositorInsertPLS-KP6
TagsHisExpressionMammalianPromoterCMVAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFPm-DAD M1041A
Plasmid#25411DepositorInsertDiap3 (Diaph3 Mouse)
TagsEGFP, His, and MycExpressionMammalianMutationAlanine substitution at critical residue in DAD t…Available SinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-sCS4-2T
Plasmid#28250DepositorInsertsingle chain Cse4-Scm3-H4 with 2 Thrombin site
TagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and Scm3. An…Available SinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
Affibody EGFR:1907.BirA*-pET301/NT-DEST
Plasmid#174691PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
TagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-I-delta N90
Plasmid#22165DepositorInsertTFII-I (GTF2I Human)
TagsGST and HisExpressionMammalianMutationThe first 90 aa of TFII-I are deletedAvailable SinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-GST-TNRC6A-(935-984)
Plasmid#42050DepositorAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
mRFP-dmBACCS2
Plasmid#67630PurposeDrosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switchDepositorInsertmRFP-dmBACCS2
UseDrosophila expressionPromoterDrosophila actin gene 5C gene promoterAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GATA1
Plasmid#67068PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL3
Plasmid#169108PurposeExpresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_T5
Plasmid#155175Purposesee pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-FOP
Plasmid#136796PurposeMammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tagDepositorInsertSNAP-FGFR
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOE5751.1
Plasmid#135083PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-His tag-eGFP
ExpressionBacterialAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSETC-PITPbeta (splice variant 1)
Plasmid#58270Purposebacterial expression of PITP beta (phosphatidylinositol transfer protein beta)DepositorAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only