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pLenti4-Puro-H19

Plasmid

#200835

Purpose

Lentiviral vector expressing human long non-coding RNA H19

Depositor

Reinier Boon

Article

Hofmann et al Cardiovasc Res. 2019 Jan 1;115(1):230-24

Insert

Human long non-coding RNA H19

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHage2-EF1a-TERT-IRES-Puro

Plasmid

#182590

Purpose

expresses TERT in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPW3758 : CMVd3-HsIRE1-HaloTag

Plasmid

#185680

Purpose

Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Human, Synthetic)

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Long P2A

Plasmid

#164115

Purpose

Detect the long Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Short P2A

Plasmid

#164116

Purpose

Detect the short Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Short P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2_EF1a_TetOn_IRES_mCherry

Plasmid

#204155

Purpose

Lentivirus backbone expressing rtTA linked to mCherry

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

Tet-On 3G

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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HDM-Hgpm2

Plasmid

#204152

Purpose

Lentivirus helper plasmid coding for Gag/Pol

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

HIV-1 gag/pol

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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HDM-tat1b

Plasmid

#204154

Purpose

Lentivirus helper plasmid coding for Tat

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

tat

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSIRV-NFAT-eGFP

Plasmid

#118031

Purpose

Fluorescent reporter for NFAT activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eGFP

Use

Retroviral

Promoter

minimal promoter with NFAT binding site

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

Plasmid

#68375

Purpose

Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Promoter

human PGK1

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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JD633

Plasmid

#160393

Purpose

CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Inserts

ZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA

Use

Binary vector

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CAG-CasRX-P2A-EGFP-BGH PolyA

Plasmid

#154004

Purpose

Express CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

CRISPR

Promoter

CAG

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAF0687 Pa01_attP-mCherry donor

Plasmid

#193466

Purpose

Promoterless mcherry donor for Pa01

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-RFPKDEL

Plasmid

#138660

Purpose

Reporter plasmid expressing the ER-located RFP

Depositor

Bertrand Collet

Article

Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

Insert

RFP-KDEL

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pXR001: EF1a-CasRx-2A-EGFP

Plasmid

#109049

Purpose

CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

CasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Promoter

EF1A

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET28HIS-hAPE1

Plasmid

#70757

Purpose

bacterial expression of human APE1

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

APEX1 (APEX1 Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSIRV-AP-1-mCherry

Plasmid

#118095

Purpose

Fluorescent reporter for AP-1 activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

mCherry

Use

Retroviral

Promoter

minimal promoter with AP-1 responsive element

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3G-p300-dCas9-P2A-mCherry

Plasmid

#138456

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

p300 core

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,5)P2 Probe

Plasmid

#211512

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SnxA-2xPX

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/EV

Plasmid

#120833

Purpose

Control vector, 20nt random sequence between gateway recombination sites

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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