We narrowed to 12,126 results for: LEA
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Plasmid#128279PurposeMammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion proteinDepositorInserthTetR::NLS::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsEGFPExpressionMammalianPromoterCMV-D2ir promoterAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSADuet148
Plasmid#121910PurposeCharge-introduced NpuDnaBΔ283ΔC39 inteinDepositorInsertCI-NpuDnaBΔ290ΔC39 intein
TagsHis6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0015
Plasmid#117551PurposeLevel 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3/gamma2SIL
Plasmid#121433PurposeGABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1/gamma2SIL
Plasmid#121432PurposeGABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-Q42L;Q123A-N’His
Plasmid#117710Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
TagsHis tag with Thrombin cleavage siteExpressionBacterialMutationQ42L; Q123APromoterT7Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0011
Plasmid#117547PurposeLevel 1 Golden Gate Cassette: eCas9 1.1 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0010
Plasmid#117546PurposeLevel 1 Golden Gate Cassette: eCas9 1.0 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.0 (no stop codon) (pEPOR0SP0017) + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0009
Plasmid#117545PurposeLevel 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-KA (no stop codon)(pEPOR0SP0015) + YFP (pICSL50005)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0008
Plasmid#117544PurposeLevel 1 Golden Gate Cassette: Cas9-DE expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-DE (no stop codon)(pEPOR0SP0001) + YFP (pICSL50005)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0002
Plasmid#117543PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-F219L (JBEI-7186)
Plasmid#110151PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant geneDepositorInsertGGR
TagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-F219L mutant genePromoterT7Available SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T-MsePCNA2
Plasmid#107064PurposeExpresses M. sedula PCNA2 in E. coliDepositorInsertM. sedula PCNA2
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T-MsePCNA1
Plasmid#107063PurposeExpresses M. sedula PCNA1 in E. coliDepositorInsertM. sedula PCNA1
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T-MsePCNA3
Plasmid#107065PurposeExpresses M. sedula PCNA3 in E. coliDepositorInsertM. sedula PCNA3
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceFeb. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663K
Plasmid#92239PurposeExpress R663K mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663K mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to LysinePromoterT7 promoterAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
2QO2 (EPHA3)
Plasmid#101271PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEPHA3 (EPHA3 Human)
TagsHexahistidine tag with Thrombin cleavageExpressionBacterialPromoterT7Available SinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
GLT-1b delta 4
Plasmid#98707Purposemammalian expression of Glt1b delta 4 mutantDepositorInsertGlt1b (Slc1a2 Rat)
ExpressionMammalianMutationmissing last 4 amino acids at the C-terminus. I52…Available SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-W695del
Plasmid#92241PurposeExpress W695-deletion mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-W695deletion mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationTryptophan 695 deletedPromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663Q
Plasmid#92240PurposeExpress R663Q mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663Q mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to GlutaminePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only