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pAAV_GR-RE_MLPmin_BC1093-luc2

Plasmid

#227113

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1093

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1093 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC109-P2B1

Plasmid

#202950

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC109

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC109

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1093-1322_S

Plasmid

#147532

Purpose

Mammalian Expression of HsNot1iso1_1093-1322

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_1093-1322 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E109_I112delinsD

Plasmid

#116468

Purpose

Lentiviral expression of PIK3CA E109_I112delinsD

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E109_I112delinsD

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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TESK1 gRNA (BRDN0001145109)

Plasmid

#77892

Purpose

3rd generation lentiviral gRNA plasmid targeting human TESK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TESK1 (TESK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRPM6 gRNA (BRDN0001148109)

Plasmid

#77336

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM6 (TRPM6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACB gRNA (BRDN0001147109)

Plasmid

#75961

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACB (PRKACB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKMT1A gRNA (BRDN0001149109)

Plasmid

#75880

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKMT1A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKMT1A,CKMT1B (CKMT1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p6109 pSG5-HPV31-E2(HA)

Plasmid

#24740

Depositor

Peter Howley

Article

Powell et al J Virol. 2010 May;84(9):4451-60. doi: 10

Insert

HPV31 E2 (HA)

Tags

HA tag in hinge region

Expression

Mammalian

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only
Ta1098 E18A/D21A

Plasmid

#19073

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

E18A/D21A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 E18A/D22A

Plasmid

#19078

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

E18A/D22A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 D21A/D22A

Plasmid

#19079

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

D21A/D22A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098(130-242)

Plasmid

#19087

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098(243-339)

Plasmid

#19088

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098(130-339)

Plasmid

#19090

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag-humanTRAF6 (1093)

Plasmid

#66929

Purpose

Mammalian Expression of TRAF6

Depositor

Michael Karin

Insert

TRAF6 (TRAF6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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1098 pcDNA GFP FKHR

Plasmid

#9022

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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LVXN-Neo-NSD2-E1099K

Plasmid

#86011

Purpose

express NSD2 E1099K mutant in mammalian cells

Depositor

Darrin Stuart

Article

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 E1099K mutant (NSD2 Human)

Tags

Flag tag D Y K D D D D K

Expression

Mammalian

Mutation

E1099K; K1002R (please see depositor comments bel…

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
pEC3109 (pSpy1C-Plac(1)_ffluc)

Plasmid

#218514

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of mrfp cassette, mutagenesis of the RBS…

Promoter

Plac(1)

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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